Working on 268_001.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 27.1606 Std = 5.6538 Hotpixel threshold = 61.0835 Detected 295 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 19.2443 px Iteration 2: RMSD = 1.30972 px Iteration 3: RMSD = 0.231248 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 4.62387 px Iteration 2: RMSD = 0.662759 px Iteration 3: RMSD = 0.142396 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 3.77263 px Iteration 2: RMSD = 1.07073 px Iteration 3: RMSD = 0.295634 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 3.78987 px Iteration 2: RMSD = 0.764995 px Iteration 3: RMSD = 0.308116 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 4.70032 px Iteration 2: RMSD = 0.981194 px Iteration 3: RMSD = 0.185781 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 4.688 px Iteration 2: RMSD = 0.772237 px Iteration 3: RMSD = 0.312675 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 4.91001 px Iteration 2: RMSD = 6.07394 px Iteration 3: RMSD = 0.684232 px Iteration 4: RMSD = 0.306172 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 7.60557 px Iteration 2: RMSD = 0.904555 px Iteration 3: RMSD = 0.562792 px Iteration 4: RMSD = 0.249721 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 5.51691 px Iteration 2: RMSD = 0.931579 px Iteration 3: RMSD = 0.321827 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 8.28748 px Iteration 2: RMSD = 3.96428 px Iteration 3: RMSD = 5.01521 px Iteration 4: RMSD = 5.0759 px Iteration 5: RMSD = 5.06703 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 4.07469 px Iteration 2: RMSD = 2.52607 px Iteration 3: RMSD = 0.768031 px Iteration 4: RMSD = 0.331825 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 7.09988 px Iteration 2: RMSD = 4.86229 px Iteration 3: RMSD = 0.793551 px Iteration 4: RMSD = 0.401388 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 15.1158 px Iteration 2: RMSD = 8.20586 px Iteration 3: RMSD = 8.85128 px Iteration 4: RMSD = 7.54406 px Iteration 5: RMSD = 9.41138 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 6.37313 px Iteration 2: RMSD = 1.69265 px Iteration 3: RMSD = 0.747961 px Iteration 4: RMSD = 0.295336 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 5.50928 px Iteration 2: RMSD = 6.84679 px Iteration 3: RMSD = 7.83522 px Iteration 4: RMSD = 1.54859 px Iteration 5: RMSD = 1.4752 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 5.2791 px Iteration 2: RMSD = 4.87236 px Iteration 3: RMSD = 4.43133 px Iteration 4: RMSD = 2.94505 px Iteration 5: RMSD = 0.790804 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 11.0416 px Iteration 2: RMSD = 9.34536 px Iteration 3: RMSD = 6.41098 px Iteration 4: RMSD = 5.11862 px Iteration 5: RMSD = 1.26639 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 18.2208 px Iteration 2: RMSD = 2.4044 px Iteration 3: RMSD = 2.27553 px Iteration 4: RMSD = 0.916838 px Iteration 5: RMSD = 0.92507 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 27.1427 px Iteration 2: RMSD = 10.9649 px Iteration 3: RMSD = 10.8896 px Iteration 4: RMSD = 7.26753 px Iteration 5: RMSD = 4.95601 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 19.3767 px Iteration 2: RMSD = 10.8972 px Iteration 3: RMSD = 7.71963 px Iteration 4: RMSD = 5.69578 px Iteration 5: RMSD = 4.54051 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 8.42454 px Iteration 2: RMSD = 1.30967 px Iteration 3: RMSD = 0.444918 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 21.5627 px Iteration 2: RMSD = 4.9545 px Iteration 3: RMSD = 1.86256 px Iteration 4: RMSD = 1.59893 px Iteration 5: RMSD = 1.44822 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 28.8538 px Iteration 2: RMSD = 16.3099 px Iteration 3: RMSD = 13.8482 px Iteration 4: RMSD = 12.1672 px Iteration 5: RMSD = 10.7327 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 32.1801 px Iteration 2: RMSD = 13.482 px Iteration 3: RMSD = 10.4645 px Iteration 4: RMSD = 9.23307 px Iteration 5: RMSD = 6.52687 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 31.5627 px Iteration 2: RMSD = 6.2886 px Iteration 3: RMSD = 4.58108 px Iteration 4: RMSD = 4.40033 px Iteration 5: RMSD = 4.16614 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 46.6662 px Iteration 2: RMSD = 4.16342 px Iteration 3: RMSD = 2.25298 px Iteration 4: RMSD = 1.86994 px Iteration 5: RMSD = 1.89571 px Polynomial fit RMSD: X = 3.30958 px Y = 2.22333 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/268_001.mrc