Working on 267-99_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 26.1744 Std = 5.66153 Hotpixel threshold = 60.1436 Detected 266 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 6.41674 px Iteration 2: RMSD = 0.460486 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 5.72414 px Iteration 2: RMSD = 0.636342 px Iteration 3: RMSD = 0.374939 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 3.00302 px Iteration 2: RMSD = 1.11783 px Iteration 3: RMSD = 1.20592 px Iteration 4: RMSD = 0.548752 px Iteration 5: RMSD = 0.30932 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 9.91699 px Iteration 2: RMSD = 3.66324 px Iteration 3: RMSD = 1.2203 px Iteration 4: RMSD = 0.488937 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 17.3193 px Iteration 2: RMSD = 10.6254 px Iteration 3: RMSD = 9.40396 px Iteration 4: RMSD = 9.78703 px Iteration 5: RMSD = 10.8624 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 16.1607 px Iteration 2: RMSD = 38.1896 px Iteration 3: RMSD = 36.8959 px Iteration 4: RMSD = 36.1388 px Iteration 5: RMSD = 37.2098 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 6.39468 px Iteration 2: RMSD = 0.823132 px Iteration 3: RMSD = 0.37306 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 8.14663 px Iteration 2: RMSD = 0.875379 px Iteration 3: RMSD = 0.478056 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 8.6992 px Iteration 2: RMSD = 1.34861 px Iteration 3: RMSD = 1.09309 px Iteration 4: RMSD = 0.358508 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 17.4305 px Iteration 2: RMSD = 8.54107 px Iteration 3: RMSD = 11.0132 px Iteration 4: RMSD = 8.99495 px Iteration 5: RMSD = 9.05532 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 23.7773 px Iteration 2: RMSD = 7.07694 px Iteration 3: RMSD = 4.75154 px Iteration 4: RMSD = 4.58242 px Iteration 5: RMSD = 1.44633 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 5.14634 px Iteration 2: RMSD = 1.01927 px Iteration 3: RMSD = 0.551938 px Iteration 4: RMSD = 0.217603 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 7.74146 px Iteration 2: RMSD = 0.719809 px Iteration 3: RMSD = 0.517434 px Iteration 4: RMSD = 0.250508 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 4.02366 px Iteration 2: RMSD = 1.34239 px Iteration 3: RMSD = 0.659992 px Iteration 4: RMSD = 0.403683 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 11.5109 px Iteration 2: RMSD = 8.79152 px Iteration 3: RMSD = 6.42136 px Iteration 4: RMSD = 6.44996 px Iteration 5: RMSD = 3.62713 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 13.5468 px Iteration 2: RMSD = 3.81886 px Iteration 3: RMSD = 3.52284 px Iteration 4: RMSD = 2.97402 px Iteration 5: RMSD = 2.86322 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 3.8488 px Iteration 2: RMSD = 0.59022 px Iteration 3: RMSD = 0.155993 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 5.71005 px Iteration 2: RMSD = 0.416413 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 5.0869 px Iteration 2: RMSD = 1.29596 px Iteration 3: RMSD = 1.01004 px Iteration 4: RMSD = 0.950618 px Iteration 5: RMSD = 0.938604 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 5.2386 px Iteration 2: RMSD = 1.29354 px Iteration 3: RMSD = 0.852961 px Iteration 4: RMSD = 0.488426 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 8.75491 px Iteration 2: RMSD = 4.04567 px Iteration 3: RMSD = 3.97005 px Iteration 4: RMSD = 3.83776 px Iteration 5: RMSD = 2.38562 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 2.79174 px Iteration 2: RMSD = 0.404533 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 7.57167 px Iteration 2: RMSD = 0.604097 px Iteration 3: RMSD = 0.159712 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 3.11038 px Iteration 2: RMSD = 0.708291 px Iteration 3: RMSD = 0.191169 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 3.94691 px Iteration 2: RMSD = 0.852651 px Iteration 3: RMSD = 0.307417 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 4.86291 px Iteration 2: RMSD = 1.86061 px Iteration 3: RMSD = 0.576477 px Iteration 4: RMSD = 0.382819 px Polynomial fit RMSD: X = 3.11359 px Y = 3.44371 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/267-99_003.mrc