Working on 267-86_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 26.5502 Std = 5.6846 Hotpixel threshold = 60.6578 Detected 277 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 15.8227 px Iteration 2: RMSD = 0.272453 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 37.488 px Iteration 2: RMSD = 11.5776 px Iteration 3: RMSD = 8.42886 px Iteration 4: RMSD = 8.928 px Iteration 5: RMSD = 6.63815 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 20.4289 px Iteration 2: RMSD = 13.3881 px Iteration 3: RMSD = 7.43367 px Iteration 4: RMSD = 6.22447 px Iteration 5: RMSD = 4.33641 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 10.7894 px Iteration 2: RMSD = 9.91617 px Iteration 3: RMSD = 7.58811 px Iteration 4: RMSD = 7.56798 px Iteration 5: RMSD = 7.51844 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 10.2838 px Iteration 2: RMSD = 1.69778 px Iteration 3: RMSD = 0.359339 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 1.50325 px Iteration 2: RMSD = 0.362845 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 29.5456 px Iteration 2: RMSD = 12.0918 px Iteration 3: RMSD = 11.9173 px Iteration 4: RMSD = 10.197 px Iteration 5: RMSD = 9.18986 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 18.3757 px Iteration 2: RMSD = 16.9923 px Iteration 3: RMSD = 19.2725 px Iteration 4: RMSD = 17.5103 px Iteration 5: RMSD = 15.5303 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 7.75961 px Iteration 2: RMSD = 2.90989 px Iteration 3: RMSD = 2.41619 px Iteration 4: RMSD = 2.23707 px Iteration 5: RMSD = 4.13996 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 5.13047 px Iteration 2: RMSD = 0.811402 px Iteration 3: RMSD = 0.138211 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 4.72777 px Iteration 2: RMSD = 0.230567 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 16.4275 px Iteration 2: RMSD = 6.20652 px Iteration 3: RMSD = 6.59261 px Iteration 4: RMSD = 7.11353 px Iteration 5: RMSD = 7.09505 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 7.90668 px Iteration 2: RMSD = 6.53984 px Iteration 3: RMSD = 7.27094 px Iteration 4: RMSD = 5.98819 px Iteration 5: RMSD = 2.23732 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 8.77761 px Iteration 2: RMSD = 4.79327 px Iteration 3: RMSD = 7.1287 px Iteration 4: RMSD = 1.21285 px Iteration 5: RMSD = 0.476097 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 9.81514 px Iteration 2: RMSD = 1.97668 px Iteration 3: RMSD = 1.48515 px Iteration 4: RMSD = 1.05377 px Iteration 5: RMSD = 0.940765 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 3.01754 px Iteration 2: RMSD = 0.824538 px Iteration 3: RMSD = 0.220458 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 9.62073 px Iteration 2: RMSD = 9.0322 px Iteration 3: RMSD = 10.84 px Iteration 4: RMSD = 10.8214 px Iteration 5: RMSD = 12.3561 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 9.98565 px Iteration 2: RMSD = 5.25222 px Iteration 3: RMSD = 4.96811 px Iteration 4: RMSD = 0.909667 px Iteration 5: RMSD = 0.612533 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 8.11754 px Iteration 2: RMSD = 2.43895 px Iteration 3: RMSD = 1.01102 px Iteration 4: RMSD = 0.369527 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 4.03313 px Iteration 2: RMSD = 0.728023 px Iteration 3: RMSD = 0.332877 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 37.7128 px Iteration 2: RMSD = 0.769215 px Iteration 3: RMSD = 0.373099 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 5.08384 px Iteration 2: RMSD = 1.29956 px Iteration 3: RMSD = 0.533048 px Iteration 4: RMSD = 0.322332 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 5.18982 px Iteration 2: RMSD = 0.572093 px Iteration 3: RMSD = 0.241299 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 3.10958 px Iteration 2: RMSD = 0.820419 px Iteration 3: RMSD = 0.321218 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 4.57197 px Iteration 2: RMSD = 1.08679 px Iteration 3: RMSD = 0.436192 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 3.34799 px Iteration 2: RMSD = 0.413535 px Polynomial fit RMSD: X = 7.86938 px Y = 10.2102 px The polynomial motion model did not explain the observation very well. Local correction is disabled for this micrograph. Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/267-86_002.mrc