Working on 267-72_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 27.9371 Std = 5.66261 Hotpixel threshold = 61.9127 Detected 279 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 7.39021 px Iteration 2: RMSD = 0.576904 px Iteration 3: RMSD = 0.229114 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 27.7286 px Iteration 2: RMSD = 40.8343 px Iteration 3: RMSD = 24.3843 px Iteration 4: RMSD = 26.0989 px Iteration 5: RMSD = 23.5097 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 49.3081 px Iteration 2: RMSD = 46.4031 px Iteration 3: RMSD = 47.468 px Iteration 4: RMSD = 23.5371 px Iteration 5: RMSD = 23.9091 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 18.353 px Iteration 2: RMSD = 17.434 px Iteration 3: RMSD = 13.9029 px Iteration 4: RMSD = 14.3264 px Iteration 5: RMSD = 13.7973 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 10.749 px Iteration 2: RMSD = 8.46467 px Iteration 3: RMSD = 9.03717 px Iteration 4: RMSD = 9.14984 px Iteration 5: RMSD = 9.19361 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 8.78715 px Iteration 2: RMSD = 3.41299 px Iteration 3: RMSD = 3.3656 px Iteration 4: RMSD = 0.440797 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 44.001 px Iteration 2: RMSD = 45.4475 px Iteration 3: RMSD = 45.7164 px Iteration 4: RMSD = 16.1982 px Iteration 5: RMSD = 14.9117 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 28.6361 px Iteration 2: RMSD = 21.1739 px Iteration 3: RMSD = 19.5158 px Iteration 4: RMSD = 17.562 px Iteration 5: RMSD = 17.1087 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 9.49378 px Iteration 2: RMSD = 6.56887 px Iteration 3: RMSD = 6.03962 px Iteration 4: RMSD = 5.9261 px Iteration 5: RMSD = 5.89458 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 12.2228 px Iteration 2: RMSD = 4.51622 px Iteration 3: RMSD = 1.60379 px Iteration 4: RMSD = 2.04252 px Iteration 5: RMSD = 1.98136 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 5.31713 px Iteration 2: RMSD = 4.687 px Iteration 3: RMSD = 4.43073 px Iteration 4: RMSD = 4.46029 px Iteration 5: RMSD = 4.46965 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 27.4596 px Iteration 2: RMSD = 23.2912 px Iteration 3: RMSD = 22.9768 px Iteration 4: RMSD = 23.3686 px Iteration 5: RMSD = 21.7393 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 25.9501 px Iteration 2: RMSD = 25.0519 px Iteration 3: RMSD = 25.6855 px Iteration 4: RMSD = 24.1884 px Iteration 5: RMSD = 22.9403 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 16.0943 px Iteration 2: RMSD = 9.63455 px Iteration 3: RMSD = 8.47405 px Iteration 4: RMSD = 8.26397 px Iteration 5: RMSD = 9.36642 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 13.2288 px Iteration 2: RMSD = 7.85063 px Iteration 3: RMSD = 7.76146 px Iteration 4: RMSD = 7.40264 px Iteration 5: RMSD = 6.62151 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 7.20827 px Iteration 2: RMSD = 5.75318 px Iteration 3: RMSD = 5.0642 px Iteration 4: RMSD = 4.99831 px Iteration 5: RMSD = 4.93226 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 69.9505 px Iteration 2: RMSD = 31.9764 px Iteration 3: RMSD = 25.2191 px Iteration 4: RMSD = 24.3543 px Iteration 5: RMSD = 23.8728 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 19.4697 px Iteration 2: RMSD = 19.9198 px Iteration 3: RMSD = 18.1074 px Iteration 4: RMSD = 17.8515 px Iteration 5: RMSD = 17.5314 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 13.1175 px Iteration 2: RMSD = 11.9946 px Iteration 3: RMSD = 12.8972 px Iteration 4: RMSD = 7.24243 px Iteration 5: RMSD = 5.01653 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 6.92161 px Iteration 2: RMSD = 6.06925 px Iteration 3: RMSD = 3.72556 px Iteration 4: RMSD = 3.15238 px Iteration 5: RMSD = 1.07119 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 6.46769 px Iteration 2: RMSD = 1.02478 px Iteration 3: RMSD = 0.567523 px Iteration 4: RMSD = 0.253082 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 22.806 px Iteration 2: RMSD = 13.8569 px Iteration 3: RMSD = 11.4311 px Iteration 4: RMSD = 10.1222 px Iteration 5: RMSD = 7.27237 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 17.963 px Iteration 2: RMSD = 15.9246 px Iteration 3: RMSD = 16.2436 px Iteration 4: RMSD = 10.4331 px Iteration 5: RMSD = 10.9834 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 13.8159 px Iteration 2: RMSD = 8.34236 px Iteration 3: RMSD = 7.33466 px Iteration 4: RMSD = 8.25963 px Iteration 5: RMSD = 8.6179 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 4.05707 px Iteration 2: RMSD = 1.35498 px Iteration 3: RMSD = 0.442045 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 5.3267 px Iteration 2: RMSD = 4.74211 px Iteration 3: RMSD = 4.35307 px Iteration 4: RMSD = 4.36553 px Iteration 5: RMSD = 4.46783 px Polynomial fit RMSD: X = 2.09923 px Y = 3.88256 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/267-72_002.mrc