Working on 267-61_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 27.7318 Std = 5.70423 Hotpixel threshold = 61.9572 Detected 208 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 4.70258 px Iteration 2: RMSD = 0.412179 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 6.74032 px Iteration 2: RMSD = 1.79709 px Iteration 3: RMSD = 1.012 px Iteration 4: RMSD = 0.543597 px Iteration 5: RMSD = 0.296827 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 12.8057 px Iteration 2: RMSD = 10.0343 px Iteration 3: RMSD = 9.55604 px Iteration 4: RMSD = 8.62048 px Iteration 5: RMSD = 5.98284 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 27.882 px Iteration 2: RMSD = 22.09 px Iteration 3: RMSD = 20.4611 px Iteration 4: RMSD = 18.0515 px Iteration 5: RMSD = 16.3718 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 43.575 px Iteration 2: RMSD = 9.88895 px Iteration 3: RMSD = 2.19461 px Iteration 4: RMSD = 1.42944 px Iteration 5: RMSD = 3.44677 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 15.3725 px Iteration 2: RMSD = 14.4214 px Iteration 3: RMSD = 13.4552 px Iteration 4: RMSD = 11.8194 px Iteration 5: RMSD = 11.721 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 3.27562 px Iteration 2: RMSD = 3.22749 px Iteration 3: RMSD = 2.96437 px Iteration 4: RMSD = 1.2023 px Iteration 5: RMSD = 0.33793 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 7.69448 px Iteration 2: RMSD = 6.54447 px Iteration 3: RMSD = 6.59959 px Iteration 4: RMSD = 1.65955 px Iteration 5: RMSD = 4.35318 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 25.5962 px Iteration 2: RMSD = 18.8055 px Iteration 3: RMSD = 16.9423 px Iteration 4: RMSD = 16.9758 px Iteration 5: RMSD = 15.1806 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 26.1857 px Iteration 2: RMSD = 44.7937 px Iteration 3: RMSD = 44.4291 px Iteration 4: RMSD = 43.6071 px Iteration 5: RMSD = 42.7309 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 13.5049 px Iteration 2: RMSD = 7.29531 px Iteration 3: RMSD = 7.07329 px Iteration 4: RMSD = 6.79041 px Iteration 5: RMSD = 6.84491 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 4.5362 px Iteration 2: RMSD = 1.0781 px Iteration 3: RMSD = 0.251745 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 3.90418 px Iteration 2: RMSD = 1.1938 px Iteration 3: RMSD = 0.373341 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 15.0974 px Iteration 2: RMSD = 12.9001 px Iteration 3: RMSD = 12.238 px Iteration 4: RMSD = 11.0329 px Iteration 5: RMSD = 11.8448 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 18.1031 px Iteration 2: RMSD = 2.74963 px Iteration 3: RMSD = 1.57385 px Iteration 4: RMSD = 1.07458 px Iteration 5: RMSD = 0.758623 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 5.90472 px Iteration 2: RMSD = 7.3777 px Iteration 3: RMSD = 2.57633 px Iteration 4: RMSD = 1.58601 px Iteration 5: RMSD = 1.20599 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 4.00998 px Iteration 2: RMSD = 0.534815 px Iteration 3: RMSD = 0.194076 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 3.71481 px Iteration 2: RMSD = 0.897953 px Iteration 3: RMSD = 0.347055 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 6.12959 px Iteration 2: RMSD = 2.67844 px Iteration 3: RMSD = 2.39603 px Iteration 4: RMSD = 1.67059 px Iteration 5: RMSD = 1.17212 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 12.1127 px Iteration 2: RMSD = 3.05362 px Iteration 3: RMSD = 2.19658 px Iteration 4: RMSD = 1.90371 px Iteration 5: RMSD = 3.70165 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 8.93284 px Iteration 2: RMSD = 1.60675 px Iteration 3: RMSD = 0.588311 px Iteration 4: RMSD = 0.270871 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 4.37447 px Iteration 2: RMSD = 0.654197 px Iteration 3: RMSD = 0.102521 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 5.91104 px Iteration 2: RMSD = 0.487026 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 3.93175 px Iteration 2: RMSD = 1.99855 px Iteration 3: RMSD = 0.981773 px Iteration 4: RMSD = 0.855997 px Iteration 5: RMSD = 0.824407 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 11.552 px Iteration 2: RMSD = 10.1969 px Iteration 3: RMSD = 5.69655 px Iteration 4: RMSD = 5.54936 px Iteration 5: RMSD = 3.52153 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 8.71411 px Iteration 2: RMSD = 0.258351 px Polynomial fit RMSD: X = 2.25917 px Y = 1.81816 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/267-61_003.mrc