Working on 267-5_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 26.0512 Std = 5.65833 Hotpixel threshold = 60.0012 Detected 238 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 3.14479 px Iteration 2: RMSD = 0.285294 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 3.84842 px Iteration 2: RMSD = 0.924946 px Iteration 3: RMSD = 0.321455 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 3.98509 px Iteration 2: RMSD = 0.622703 px Iteration 3: RMSD = 0.404958 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 3.44483 px Iteration 2: RMSD = 0.417912 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 2.4082 px Iteration 2: RMSD = 0.825418 px Iteration 3: RMSD = 0.273423 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 15.2046 px Iteration 2: RMSD = 12.499 px Iteration 3: RMSD = 11.6687 px Iteration 4: RMSD = 12.3336 px Iteration 5: RMSD = 12.4079 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 2.52195 px Iteration 2: RMSD = 0.550928 px Iteration 3: RMSD = 0.235733 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 2.81511 px Iteration 2: RMSD = 0.733958 px Iteration 3: RMSD = 0.354197 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 12.8334 px Iteration 2: RMSD = 9.61347 px Iteration 3: RMSD = 11.5937 px Iteration 4: RMSD = 11.0681 px Iteration 5: RMSD = 10.4806 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 14.4415 px Iteration 2: RMSD = 12.1644 px Iteration 3: RMSD = 13.6213 px Iteration 4: RMSD = 11.6813 px Iteration 5: RMSD = 9.57935 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 18.8225 px Iteration 2: RMSD = 23.73 px Iteration 3: RMSD = 16.3709 px Iteration 4: RMSD = 14.5374 px Iteration 5: RMSD = 12.7078 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 2.69899 px Iteration 2: RMSD = 0.745385 px Iteration 3: RMSD = 0.310289 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 3.29109 px Iteration 2: RMSD = 0.766011 px Iteration 3: RMSD = 0.444191 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 4.67652 px Iteration 2: RMSD = 0.849705 px Iteration 3: RMSD = 0.318288 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 14.5097 px Iteration 2: RMSD = 10.5657 px Iteration 3: RMSD = 11.2838 px Iteration 4: RMSD = 10.0141 px Iteration 5: RMSD = 7.44832 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 16.4069 px Iteration 2: RMSD = 11.0341 px Iteration 3: RMSD = 9.3676 px Iteration 4: RMSD = 7.63756 px Iteration 5: RMSD = 6.07028 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 6.19562 px Iteration 2: RMSD = 0.6149 px Iteration 3: RMSD = 0.221285 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 7.37741 px Iteration 2: RMSD = 7.20558 px Iteration 3: RMSD = 7.28163 px Iteration 4: RMSD = 1.0461 px Iteration 5: RMSD = 0.959638 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 3.48403 px Iteration 2: RMSD = 0.895764 px Iteration 3: RMSD = 0.549448 px Iteration 4: RMSD = 0.371415 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 8.07755 px Iteration 2: RMSD = 3.44351 px Iteration 3: RMSD = 4.62483 px Iteration 4: RMSD = 2.65991 px Iteration 5: RMSD = 0.75236 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 12.7688 px Iteration 2: RMSD = 11.6348 px Iteration 3: RMSD = 11.2357 px Iteration 4: RMSD = 11.8453 px Iteration 5: RMSD = 11.834 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 44.456 px Iteration 2: RMSD = 3.49772 px Iteration 3: RMSD = 0.860022 px Iteration 4: RMSD = 0.449741 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 32.9296 px Iteration 2: RMSD = 6.22304 px Iteration 3: RMSD = 6.79307 px Iteration 4: RMSD = 7.32424 px Iteration 5: RMSD = 7.20429 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 2.20956 px Iteration 2: RMSD = 0.514921 px Iteration 3: RMSD = 0.159307 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 3.26137 px Iteration 2: RMSD = 0.777017 px Iteration 3: RMSD = 0.391215 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 4.64124 px Iteration 2: RMSD = 3.34063 px Iteration 3: RMSD = 3.25251 px Iteration 4: RMSD = 3.23833 px Iteration 5: RMSD = 0.459219 px Polynomial fit RMSD: X = 6.97362 px Y = 4.27232 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/267-5_003.mrc