Working on 254-114_001.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 27.9452 Std = 5.63051 Hotpixel threshold = 61.7283 Detected 272 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 4.48336 px Iteration 2: RMSD = 3.15014 px Iteration 3: RMSD = 2.25958 px Iteration 4: RMSD = 1.73089 px Iteration 5: RMSD = 1.22292 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 7.11688 px Iteration 2: RMSD = 1.51131 px Iteration 3: RMSD = 0.562055 px Iteration 4: RMSD = 0.330082 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 7.02784 px Iteration 2: RMSD = 7.0831 px Iteration 3: RMSD = 7.11049 px Iteration 4: RMSD = 5.66411 px Iteration 5: RMSD = 0.420322 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 6.82435 px Iteration 2: RMSD = 1.20566 px Iteration 3: RMSD = 0.306992 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 10.4732 px Iteration 2: RMSD = 1.21984 px Iteration 3: RMSD = 0.463155 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 10.4318 px Iteration 2: RMSD = 2.90636 px Iteration 3: RMSD = 0.845985 px Iteration 4: RMSD = 0.264648 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 4.33398 px Iteration 2: RMSD = 2.78761 px Iteration 3: RMSD = 1.13274 px Iteration 4: RMSD = 0.78099 px Iteration 5: RMSD = 0.592624 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 6.5006 px Iteration 2: RMSD = 5.37192 px Iteration 3: RMSD = 0.967176 px Iteration 4: RMSD = 0.728582 px Iteration 5: RMSD = 0.264496 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 7.13348 px Iteration 2: RMSD = 5.30167 px Iteration 3: RMSD = 4.93213 px Iteration 4: RMSD = 5.04835 px Iteration 5: RMSD = 4.96509 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 13.0702 px Iteration 2: RMSD = 9.93853 px Iteration 3: RMSD = 8.46746 px Iteration 4: RMSD = 7.42055 px Iteration 5: RMSD = 7.34315 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 7.66157 px Iteration 2: RMSD = 2.50819 px Iteration 3: RMSD = 0.705701 px Iteration 4: RMSD = 0.31962 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 3.46156 px Iteration 2: RMSD = 0.99068 px Iteration 3: RMSD = 0.583867 px Iteration 4: RMSD = 0.348928 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 14.6241 px Iteration 2: RMSD = 11.9643 px Iteration 3: RMSD = 7.45298 px Iteration 4: RMSD = 4.08585 px Iteration 5: RMSD = 4.4121 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 18.878 px Iteration 2: RMSD = 13.7833 px Iteration 3: RMSD = 9.78408 px Iteration 4: RMSD = 8.61543 px Iteration 5: RMSD = 9.65511 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 10.1693 px Iteration 2: RMSD = 6.71916 px Iteration 3: RMSD = 3.40385 px Iteration 4: RMSD = 3.58728 px Iteration 5: RMSD = 3.22587 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 12.7252 px Iteration 2: RMSD = 3.1942 px Iteration 3: RMSD = 2.25488 px Iteration 4: RMSD = 1.95721 px Iteration 5: RMSD = 1.86833 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 9.20035 px Iteration 2: RMSD = 4.66314 px Iteration 3: RMSD = 2.47499 px Iteration 4: RMSD = 2.55606 px Iteration 5: RMSD = 1.55698 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 11.7218 px Iteration 2: RMSD = 6.54371 px Iteration 3: RMSD = 9.32543 px Iteration 4: RMSD = 9.12929 px Iteration 5: RMSD = 3.72818 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 48.4444 px Iteration 2: RMSD = 40.0471 px Iteration 3: RMSD = 39.133 px Iteration 4: RMSD = 34.9968 px Iteration 5: RMSD = 31.8555 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 24.3008 px Iteration 2: RMSD = 17.0248 px Iteration 3: RMSD = 16.8158 px Iteration 4: RMSD = 14.0796 px Iteration 5: RMSD = 10.6867 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 24.3523 px Iteration 2: RMSD = 18.3499 px Iteration 3: RMSD = 16.9454 px Iteration 4: RMSD = 13.7996 px Iteration 5: RMSD = 14.5013 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 6.08467 px Iteration 2: RMSD = 5.25633 px Iteration 3: RMSD = 1.35099 px Iteration 4: RMSD = 0.917805 px Iteration 5: RMSD = 0.681744 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 9.75019 px Iteration 2: RMSD = 5.84847 px Iteration 3: RMSD = 4.53872 px Iteration 4: RMSD = 3.3496 px Iteration 5: RMSD = 1.72493 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 37.4173 px Iteration 2: RMSD = 18.122 px Iteration 3: RMSD = 15.2078 px Iteration 4: RMSD = 14.5478 px Iteration 5: RMSD = 12.4843 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 38.3639 px Iteration 2: RMSD = 31.2844 px Iteration 3: RMSD = 49.7409 px Iteration 4: RMSD = 49.6124 px Iteration 5: RMSD = 49.4589 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 31.6654 px Iteration 2: RMSD = 18.6765 px Iteration 3: RMSD = 16.449 px Iteration 4: RMSD = 15.3964 px Iteration 5: RMSD = 13.9284 px Polynomial fit RMSD: X = 2.56493 px Y = 1.94699 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/254-114_001.mrc