Working on 254-108_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 27.6939 Std = 5.64189 Hotpixel threshold = 61.5453 Detected 260 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 10.7633 px Iteration 2: RMSD = 0.44311 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 28.4015 px Iteration 2: RMSD = 1.80609 px Iteration 3: RMSD = 1.2293 px Iteration 4: RMSD = 0.521741 px Iteration 5: RMSD = 0.784804 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 52.1243 px Iteration 2: RMSD = 39.773 px Iteration 3: RMSD = 9.70115 px Iteration 4: RMSD = 5.3458 px Iteration 5: RMSD = 5.35465 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 5.83829 px Iteration 2: RMSD = 1.46595 px Iteration 3: RMSD = 5.78185 px Iteration 4: RMSD = 0.48811 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 6.81603 px Iteration 2: RMSD = 1.17604 px Iteration 3: RMSD = 0.458993 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 3.35772 px Iteration 2: RMSD = 0.682839 px Iteration 3: RMSD = 0.217855 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 21.5222 px Iteration 2: RMSD = 6.82532 px Iteration 3: RMSD = 6.54486 px Iteration 4: RMSD = 6.6439 px Iteration 5: RMSD = 6.47171 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 22.1855 px Iteration 2: RMSD = 16.3647 px Iteration 3: RMSD = 11.7032 px Iteration 4: RMSD = 9.59739 px Iteration 5: RMSD = 9.41603 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 5.12855 px Iteration 2: RMSD = 1.19687 px Iteration 3: RMSD = 0.878451 px Iteration 4: RMSD = 0.49713 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 7.02876 px Iteration 2: RMSD = 1.13001 px Iteration 3: RMSD = 0.526482 px Iteration 4: RMSD = 0.422949 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 3.7733 px Iteration 2: RMSD = 1.43014 px Iteration 3: RMSD = 0.578613 px Iteration 4: RMSD = 0.195751 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 17.6705 px Iteration 2: RMSD = 40.8057 px Iteration 3: RMSD = 40.1368 px Iteration 4: RMSD = 40.7262 px Iteration 5: RMSD = 40.9985 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 9.41535 px Iteration 2: RMSD = 6.1305 px Iteration 3: RMSD = 5.10564 px Iteration 4: RMSD = 4.81908 px Iteration 5: RMSD = 4.83466 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 6.40268 px Iteration 2: RMSD = 4.02487 px Iteration 3: RMSD = 0.748056 px Iteration 4: RMSD = 1.06143 px Iteration 5: RMSD = 1.0137 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 7.3866 px Iteration 2: RMSD = 1.25998 px Iteration 3: RMSD = 0.453528 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 2.01575 px Iteration 2: RMSD = 0.716176 px Iteration 3: RMSD = 0.192606 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 10.2089 px Iteration 2: RMSD = 6.97489 px Iteration 3: RMSD = 7.36325 px Iteration 4: RMSD = 0.928865 px Iteration 5: RMSD = 0.437116 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 7.71168 px Iteration 2: RMSD = 1.25496 px Iteration 3: RMSD = 0.957893 px Iteration 4: RMSD = 0.822731 px Iteration 5: RMSD = 0.493302 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 8.47404 px Iteration 2: RMSD = 1.08823 px Iteration 3: RMSD = 0.405256 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 12.4342 px Iteration 2: RMSD = 0.920773 px Iteration 3: RMSD = 0.50012 px Iteration 4: RMSD = 0.171932 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 3.22827 px Iteration 2: RMSD = 0.722592 px Iteration 3: RMSD = 0.251951 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 7.0544 px Iteration 2: RMSD = 1.89928 px Iteration 3: RMSD = 2.62659 px Iteration 4: RMSD = 7.51772 px Iteration 5: RMSD = 7.53786 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 12.4389 px Iteration 2: RMSD = 3.15253 px Iteration 3: RMSD = 2.8692 px Iteration 4: RMSD = 2.73709 px Iteration 5: RMSD = 2.72173 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 8.0617 px Iteration 2: RMSD = 4.89869 px Iteration 3: RMSD = 0.866847 px Iteration 4: RMSD = 0.40651 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 6.62752 px Iteration 2: RMSD = 0.863424 px Iteration 3: RMSD = 0.345739 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 1.98992 px Iteration 2: RMSD = 0.394673 px Polynomial fit RMSD: X = 5.29981 px Y = 3.81311 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/254-108_002.mrc