Working on 254-101_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 28.2308 Std = 5.6113 Hotpixel threshold = 61.8986 Detected 314 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 5.04558 px Iteration 2: RMSD = 0.3729 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 24.076 px Iteration 2: RMSD = 1.91513 px Iteration 3: RMSD = 1.5007 px Iteration 4: RMSD = 0.618542 px Iteration 5: RMSD = 0.466648 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 15.3088 px Iteration 2: RMSD = 5.95967 px Iteration 3: RMSD = 1.00849 px Iteration 4: RMSD = 0.553134 px Iteration 5: RMSD = 0.189285 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 12.0718 px Iteration 2: RMSD = 7.52067 px Iteration 3: RMSD = 6.17786 px Iteration 4: RMSD = 0.563872 px Iteration 5: RMSD = 0.231501 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 9.0612 px Iteration 2: RMSD = 0.888838 px Iteration 3: RMSD = 0.376582 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 7.00948 px Iteration 2: RMSD = 0.657902 px Iteration 3: RMSD = 0.263014 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 20.6043 px Iteration 2: RMSD = 1.17651 px Iteration 3: RMSD = 0.486188 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 15.035 px Iteration 2: RMSD = 1.3628 px Iteration 3: RMSD = 0.616226 px Iteration 4: RMSD = 0.320537 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 11.3146 px Iteration 2: RMSD = 6.75789 px Iteration 3: RMSD = 6.71125 px Iteration 4: RMSD = 6.21248 px Iteration 5: RMSD = 6.19927 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 12.0967 px Iteration 2: RMSD = 6.2056 px Iteration 3: RMSD = 5.7105 px Iteration 4: RMSD = 5.68618 px Iteration 5: RMSD = 5.67707 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 6.29712 px Iteration 2: RMSD = 1.46842 px Iteration 3: RMSD = 0.473416 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 18.2339 px Iteration 2: RMSD = 8.32293 px Iteration 3: RMSD = 12.2398 px Iteration 4: RMSD = 9.44495 px Iteration 5: RMSD = 10.1929 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 14.5918 px Iteration 2: RMSD = 9.3657 px Iteration 3: RMSD = 7.24366 px Iteration 4: RMSD = 9.13545 px Iteration 5: RMSD = 9.07757 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 7.90699 px Iteration 2: RMSD = 1.61633 px Iteration 3: RMSD = 0.753184 px Iteration 4: RMSD = 0.393599 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 8.77593 px Iteration 2: RMSD = 1.1828 px Iteration 3: RMSD = 0.58499 px Iteration 4: RMSD = 0.465841 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 10.8198 px Iteration 2: RMSD = 0.83574 px Iteration 3: RMSD = 0.15911 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 13.0649 px Iteration 2: RMSD = 2.17083 px Iteration 3: RMSD = 0.939732 px Iteration 4: RMSD = 0.478946 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 8.1662 px Iteration 2: RMSD = 5.41603 px Iteration 3: RMSD = 9.16558 px Iteration 4: RMSD = 10.8963 px Iteration 5: RMSD = 10.7237 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 5.60431 px Iteration 2: RMSD = 1.69995 px Iteration 3: RMSD = 0.742393 px Iteration 4: RMSD = 0.44046 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 13.4672 px Iteration 2: RMSD = 0.974077 px Iteration 3: RMSD = 0.38464 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 6.59527 px Iteration 2: RMSD = 1.63784 px Iteration 3: RMSD = 0.574263 px Iteration 4: RMSD = 0.13882 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 3.96934 px Iteration 2: RMSD = 1.20417 px Iteration 3: RMSD = 0.48063 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 11.5761 px Iteration 2: RMSD = 5.89274 px Iteration 3: RMSD = 1.84303 px Iteration 4: RMSD = 2.27339 px Iteration 5: RMSD = 0.62002 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 13.1485 px Iteration 2: RMSD = 1.35494 px Iteration 3: RMSD = 0.669975 px Iteration 4: RMSD = 0.203035 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 9.55091 px Iteration 2: RMSD = 1.06194 px Iteration 3: RMSD = 0.462255 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 6.73627 px Iteration 2: RMSD = 0.525451 px Iteration 3: RMSD = 0.328974 px Polynomial fit RMSD: X = 4.52087 px Y = 3.36194 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/254-101_002.mrc