Working on 252_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 28.7526 Std = 5.66631 Hotpixel threshold = 62.7504 Detected 308 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 1.21125 px Iteration 2: RMSD = 0.192672 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 6.92123 px Iteration 2: RMSD = 4.90297 px Iteration 3: RMSD = 3.01021 px Iteration 4: RMSD = 1.05884 px Iteration 5: RMSD = 0.847537 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 11.1893 px Iteration 2: RMSD = 5.16558 px Iteration 3: RMSD = 6.31177 px Iteration 4: RMSD = 6.42759 px Iteration 5: RMSD = 6.44812 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 11.1319 px Iteration 2: RMSD = 4.32711 px Iteration 3: RMSD = 1.29962 px Iteration 4: RMSD = 0.343518 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 13.9008 px Iteration 2: RMSD = 6.00222 px Iteration 3: RMSD = 4.35293 px Iteration 4: RMSD = 4.19156 px Iteration 5: RMSD = 4.11581 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 9.73987 px Iteration 2: RMSD = 2.44275 px Iteration 3: RMSD = 1.31732 px Iteration 4: RMSD = 0.790308 px Iteration 5: RMSD = 0.570595 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 6.72226 px Iteration 2: RMSD = 1.07155 px Iteration 3: RMSD = 0.360887 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 9.23032 px Iteration 2: RMSD = 10.7798 px Iteration 3: RMSD = 8.80196 px Iteration 4: RMSD = 8.74279 px Iteration 5: RMSD = 8.19017 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 12.3723 px Iteration 2: RMSD = 6.06422 px Iteration 3: RMSD = 2.664 px Iteration 4: RMSD = 2.45623 px Iteration 5: RMSD = 2.32978 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 12.7732 px Iteration 2: RMSD = 9.65692 px Iteration 3: RMSD = 4.57876 px Iteration 4: RMSD = 3.64646 px Iteration 5: RMSD = 3.79937 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 15.4809 px Iteration 2: RMSD = 6.33521 px Iteration 3: RMSD = 6.28598 px Iteration 4: RMSD = 5.9424 px Iteration 5: RMSD = 4.97484 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 6.71619 px Iteration 2: RMSD = 1.02259 px Iteration 3: RMSD = 0.52241 px Iteration 4: RMSD = 0.247894 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 3.53583 px Iteration 2: RMSD = 0.970059 px Iteration 3: RMSD = 0.642874 px Iteration 4: RMSD = 0.439598 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 9.64674 px Iteration 2: RMSD = 9.85613 px Iteration 3: RMSD = 8.33254 px Iteration 4: RMSD = 4.06406 px Iteration 5: RMSD = 3.62182 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 14.8831 px Iteration 2: RMSD = 11.7636 px Iteration 3: RMSD = 13.0722 px Iteration 4: RMSD = 8.6918 px Iteration 5: RMSD = 8.21344 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 15.4809 px Iteration 2: RMSD = 5.31325 px Iteration 3: RMSD = 5.14213 px Iteration 4: RMSD = 4.93479 px Iteration 5: RMSD = 5.04173 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 4.5662 px Iteration 2: RMSD = 0.638588 px Iteration 3: RMSD = 0.21145 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 8.97484 px Iteration 2: RMSD = 6.16812 px Iteration 3: RMSD = 6.00702 px Iteration 4: RMSD = 6.18388 px Iteration 5: RMSD = 6.23861 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 4.61513 px Iteration 2: RMSD = 0.915637 px Iteration 3: RMSD = 0.429569 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 16.6289 px Iteration 2: RMSD = 13.259 px Iteration 3: RMSD = 13.7473 px Iteration 4: RMSD = 13.5399 px Iteration 5: RMSD = 12.0949 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 22.0421 px Iteration 2: RMSD = 20.762 px Iteration 3: RMSD = 19.9627 px Iteration 4: RMSD = 19.8596 px Iteration 5: RMSD = 18.147 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 4.10755 px Iteration 2: RMSD = 0.725572 px Iteration 3: RMSD = 0.258136 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 4.92608 px Iteration 2: RMSD = 0.647513 px Iteration 3: RMSD = 0.323449 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 7.57107 px Iteration 2: RMSD = 6.39497 px Iteration 3: RMSD = 6.36246 px Iteration 4: RMSD = 6.17228 px Iteration 5: RMSD = 6.1842 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 11.5257 px Iteration 2: RMSD = 4.75147 px Iteration 3: RMSD = 4.55304 px Iteration 4: RMSD = 0.468958 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 9.44229 px Iteration 2: RMSD = 1.34362 px Iteration 3: RMSD = 0.523488 px Iteration 4: RMSD = 0.27519 px Polynomial fit RMSD: X = 3.58685 px Y = 3.90618 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/252_003.mrc