Working on 252_002.tif with 10 thread(s).

Movie size: X = 3838 Y = 3710 N = 80
Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80
Frame grouping: n_frames = 80, requested group size = 1
 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 
interpolate_shifts = 0

In unaligned sum, Mean = 28.7101 Std = 5.64053 Hotpixel threshold = 62.5533
Detected 331 hot pixels to be corrected.
Fixed hot pixels.

Global alignment:
 Iteration 1: RMSD = 5.02928 px
 Iteration 2: RMSD = 0.276817 px

Local alignments:
Patches: X = 5 Y = 5
Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370)
 Iteration 1: RMSD = 23.1397 px
 Iteration 2: RMSD = 9.37007 px
 Iteration 3: RMSD = 5.91297 px
 Iteration 4: RMSD = 4.66626 px
 Iteration 5: RMSD = 4.65221 px
Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370)
 Iteration 1: RMSD = 14.6386 px
 Iteration 2: RMSD = 1.90072 px
 Iteration 3: RMSD = 0.987366 px
 Iteration 4: RMSD = 0.579916 px
 Iteration 5: RMSD = 0.335497 px
Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370)
 Iteration 1: RMSD = 9.07257 px
 Iteration 2: RMSD = 4.10991 px
 Iteration 3: RMSD = 4.15346 px
 Iteration 4: RMSD = 4.0914 px
 Iteration 5: RMSD = 4.08617 px
Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370)
 Iteration 1: RMSD = 9.36309 px
 Iteration 2: RMSD = 8.37281 px
 Iteration 3: RMSD = 1.67371 px
 Iteration 4: RMSD = 1.67346 px
 Iteration 5: RMSD = 1.66944 px
Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370)
 Iteration 1: RMSD = 9.9391 px
 Iteration 2: RMSD = 1.54199 px
 Iteration 3: RMSD = 0.82697 px
 Iteration 4: RMSD = 0.25017 px
Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112)
 Iteration 1: RMSD = 17.5272 px
 Iteration 2: RMSD = 4.52352 px
 Iteration 3: RMSD = 4.49784 px
 Iteration 4: RMSD = 4.36098 px
 Iteration 5: RMSD = 4.43626 px
Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112)
 Iteration 1: RMSD = 11.5698 px
 Iteration 2: RMSD = 2.27559 px
 Iteration 3: RMSD = 5.0542 px
 Iteration 4: RMSD = 6.00803 px
 Iteration 5: RMSD = 0.434434 px
Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112)
 Iteration 1: RMSD = 5.59823 px
 Iteration 2: RMSD = 0.945975 px
 Iteration 3: RMSD = 0.304782 px
Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112)
 Iteration 1: RMSD = 28.5346 px
 Iteration 2: RMSD = 2.06844 px
 Iteration 3: RMSD = 0.631811 px
 Iteration 4: RMSD = 0.423432 px
Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112)
 Iteration 1: RMSD = 7.70547 px
 Iteration 2: RMSD = 1.35432 px
 Iteration 3: RMSD = 0.427747 px
Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854)
 Iteration 1: RMSD = 16.3878 px
 Iteration 2: RMSD = 3.68785 px
 Iteration 3: RMSD = 2.41634 px
 Iteration 4: RMSD = 0.958958 px
 Iteration 5: RMSD = 0.793389 px
Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854)
 Iteration 1: RMSD = 6.86889 px
 Iteration 2: RMSD = 7.67415 px
 Iteration 3: RMSD = 7.87079 px
 Iteration 4: RMSD = 5.17764 px
 Iteration 5: RMSD = 5.03784 px
Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854)
 Iteration 1: RMSD = 34.0388 px
 Iteration 2: RMSD = 12.1289 px
 Iteration 3: RMSD = 11.8478 px
 Iteration 4: RMSD = 11.851 px
 Iteration 5: RMSD = 9.69608 px
Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854)
 Iteration 1: RMSD = 10.779 px
 Iteration 2: RMSD = 3.9292 px
 Iteration 3: RMSD = 5.22438 px
 Iteration 4: RMSD = 5.28126 px
 Iteration 5: RMSD = 5.31527 px
Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854)
 Iteration 1: RMSD = 7.10492 px
 Iteration 2: RMSD = 0.649516 px
 Iteration 3: RMSD = 0.248966 px
Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596)
 Iteration 1: RMSD = 12.5204 px
 Iteration 2: RMSD = 6.59869 px
 Iteration 3: RMSD = 3.82127 px
 Iteration 4: RMSD = 1.7163 px
 Iteration 5: RMSD = 0.651308 px
Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596)
 Iteration 1: RMSD = 10.0578 px
 Iteration 2: RMSD = 9.2326 px
 Iteration 3: RMSD = 5.61099 px
 Iteration 4: RMSD = 1.97176 px
 Iteration 5: RMSD = 1.25173 px
Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596)
 Iteration 1: RMSD = 11.3682 px
 Iteration 2: RMSD = 5.75847 px
 Iteration 3: RMSD = 5.58023 px
 Iteration 4: RMSD = 2.15817 px
 Iteration 5: RMSD = 6.13049 px
Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596)
 Iteration 1: RMSD = 12.7621 px
 Iteration 2: RMSD = 0.98747 px
 Iteration 3: RMSD = 0.32274 px
Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596)
 Iteration 1: RMSD = 5.23828 px
 Iteration 2: RMSD = 0.386463 px
Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338)
 Iteration 1: RMSD = 4.29602 px
 Iteration 2: RMSD = 1.25083 px
 Iteration 3: RMSD = 0.403308 px
Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338)
 Iteration 1: RMSD = 45.8493 px
 Iteration 2: RMSD = 8.98213 px
 Iteration 3: RMSD = 5.7536 px
 Iteration 4: RMSD = 3.41729 px
 Iteration 5: RMSD = 3.47208 px
Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338)
 Iteration 1: RMSD = 13.5767 px
 Iteration 2: RMSD = 8.31804 px
 Iteration 3: RMSD = 4.3563 px
 Iteration 4: RMSD = 4.49943 px
 Iteration 5: RMSD = 4.396 px
Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338)
 Iteration 1: RMSD = 8.85209 px
 Iteration 2: RMSD = 1.10286 px
 Iteration 3: RMSD = 0.385827 px
Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338)
 Iteration 1: RMSD = 3.82989 px
 Iteration 2: RMSD = 0.385512 px

Polynomial fit RMSD: X = 7.64113 px Y = 10.8622 px
The polynomial motion model did not explain the observation very well.
Local correction is disabled for this micrograph.
Summing frames after dose weighting: ................................................................................ done
Written aligned and dose-weighted sum to MotionCorr/job002/252_002.mrc