Working on 252_001.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 29.2442 Std = 5.62301 Hotpixel threshold = 62.9823 Detected 327 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 3.65685 px Iteration 2: RMSD = 0.88227 px Iteration 3: RMSD = 0.337378 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 12.5535 px Iteration 2: RMSD = 1.97789 px Iteration 3: RMSD = 0.482676 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 8.54173 px Iteration 2: RMSD = 1.23861 px Iteration 3: RMSD = 0.240518 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 10.8905 px Iteration 2: RMSD = 1.85232 px Iteration 3: RMSD = 0.592792 px Iteration 4: RMSD = 0.268104 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 9.66417 px Iteration 2: RMSD = 1.17936 px Iteration 3: RMSD = 0.618904 px Iteration 4: RMSD = 0.364755 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 12.854 px Iteration 2: RMSD = 1.10553 px Iteration 3: RMSD = 0.394419 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 12.228 px Iteration 2: RMSD = 6.15377 px Iteration 3: RMSD = 2.64574 px Iteration 4: RMSD = 1.69358 px Iteration 5: RMSD = 1.46771 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 9.20055 px Iteration 2: RMSD = 9.29609 px Iteration 3: RMSD = 7.5693 px Iteration 4: RMSD = 7.64186 px Iteration 5: RMSD = 7.23966 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 8.291 px Iteration 2: RMSD = 1.97153 px Iteration 3: RMSD = 1.8482 px Iteration 4: RMSD = 1.35412 px Iteration 5: RMSD = 1.08675 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 12.4149 px Iteration 2: RMSD = 4.24708 px Iteration 3: RMSD = 0.824773 px Iteration 4: RMSD = 0.713931 px Iteration 5: RMSD = 0.292745 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 6.56521 px Iteration 2: RMSD = 2.7121 px Iteration 3: RMSD = 0.538206 px Iteration 4: RMSD = 0.191421 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 15.0703 px Iteration 2: RMSD = 5.75936 px Iteration 3: RMSD = 0.79032 px Iteration 4: RMSD = 0.437586 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 25.2846 px Iteration 2: RMSD = 12.8626 px Iteration 3: RMSD = 8.01712 px Iteration 4: RMSD = 7.58227 px Iteration 5: RMSD = 7.52985 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 24.7857 px Iteration 2: RMSD = 13.6265 px Iteration 3: RMSD = 8.55713 px Iteration 4: RMSD = 7.70721 px Iteration 5: RMSD = 6.2291 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 18.3371 px Iteration 2: RMSD = 5.66989 px Iteration 3: RMSD = 5.03583 px Iteration 4: RMSD = 5.15551 px Iteration 5: RMSD = 5.14583 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 12.6899 px Iteration 2: RMSD = 4.67756 px Iteration 3: RMSD = 1.37586 px Iteration 4: RMSD = 0.644024 px Iteration 5: RMSD = 0.342818 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 28.8382 px Iteration 2: RMSD = 5.90827 px Iteration 3: RMSD = 4.97093 px Iteration 4: RMSD = 1.17259 px Iteration 5: RMSD = 0.74409 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 37.6461 px Iteration 2: RMSD = 7.70122 px Iteration 3: RMSD = 9.80123 px Iteration 4: RMSD = 6.22078 px Iteration 5: RMSD = 5.35853 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 56.5691 px Iteration 2: RMSD = 44.2451 px Iteration 3: RMSD = 43.0731 px Iteration 4: RMSD = 40.4552 px Iteration 5: RMSD = 40.3733 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 40.4772 px Iteration 2: RMSD = 10.6183 px Iteration 3: RMSD = 5.81738 px Iteration 4: RMSD = 5.60276 px Iteration 5: RMSD = 5.69369 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 17.2889 px Iteration 2: RMSD = 6.84552 px Iteration 3: RMSD = 5.09084 px Iteration 4: RMSD = 4.95984 px Iteration 5: RMSD = 5.05785 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 19.2876 px Iteration 2: RMSD = 43.2539 px Iteration 3: RMSD = 4.21692 px Iteration 4: RMSD = 3.92825 px Iteration 5: RMSD = 3.89481 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 49.1085 px Iteration 2: RMSD = 4.93963 px Iteration 3: RMSD = 4.01067 px Iteration 4: RMSD = 4.16962 px Iteration 5: RMSD = 3.93802 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 45.8783 px Iteration 2: RMSD = 7.86608 px Iteration 3: RMSD = 1.28968 px Iteration 4: RMSD = 0.353548 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 49.5074 px Iteration 2: RMSD = 6.1532 px Iteration 3: RMSD = 6.92608 px Iteration 4: RMSD = 6.84495 px Iteration 5: RMSD = 6.86138 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 20.3045 px Iteration 2: RMSD = 22.3566 px Iteration 3: RMSD = 21.8579 px Iteration 4: RMSD = 21.69 px Iteration 5: RMSD = 21.698 px Polynomial fit RMSD: X = 11.1315 px Y = 5.45781 px The polynomial motion model did not explain the observation very well. Local correction is disabled for this micrograph. Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/252_001.mrc