Working on 249_002.tif with 10 thread(s).

Movie size: X = 3838 Y = 3710 N = 80
Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80
Frame grouping: n_frames = 80, requested group size = 1
 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 
interpolate_shifts = 0

In unaligned sum, Mean = 27.8988 Std = 5.63218 Hotpixel threshold = 61.6919
Detected 294 hot pixels to be corrected.
Fixed hot pixels.

Global alignment:
 Iteration 1: RMSD = 11.2075 px
 Iteration 2: RMSD = 0.737316 px
 Iteration 3: RMSD = 0.0455018 px

Local alignments:
Patches: X = 5 Y = 5
Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370)
 Iteration 1: RMSD = 21.2487 px
 Iteration 2: RMSD = 4.51969 px
 Iteration 3: RMSD = 2.40984 px
 Iteration 4: RMSD = 2.1116 px
 Iteration 5: RMSD = 2.05697 px
Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370)
 Iteration 1: RMSD = 21.1427 px
 Iteration 2: RMSD = 8.24955 px
 Iteration 3: RMSD = 5.14115 px
 Iteration 4: RMSD = 4.81544 px
 Iteration 5: RMSD = 0.909113 px
Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370)
 Iteration 1: RMSD = 2.98674 px
 Iteration 2: RMSD = 1.19491 px
 Iteration 3: RMSD = 0.654692 px
 Iteration 4: RMSD = 0.572915 px
 Iteration 5: RMSD = 0.357866 px
Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370)
 Iteration 1: RMSD = 7.38819 px
 Iteration 2: RMSD = 1.30813 px
 Iteration 3: RMSD = 0.398279 px
Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370)
 Iteration 1: RMSD = 1.81228 px
 Iteration 2: RMSD = 0.991604 px
 Iteration 3: RMSD = 0.348993 px
Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112)
 Iteration 1: RMSD = 61.2266 px
 Iteration 2: RMSD = 12.4824 px
 Iteration 3: RMSD = 8.59077 px
 Iteration 4: RMSD = 7.29036 px
 Iteration 5: RMSD = 7.22871 px
Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112)
 Iteration 1: RMSD = 13.0224 px
 Iteration 2: RMSD = 8.09346 px
 Iteration 3: RMSD = 7.1466 px
 Iteration 4: RMSD = 5.27661 px
 Iteration 5: RMSD = 5.26253 px
Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112)
 Iteration 1: RMSD = 41.5341 px
 Iteration 2: RMSD = 1.81912 px
 Iteration 3: RMSD = 2.28407 px
 Iteration 4: RMSD = 0.735113 px
 Iteration 5: RMSD = 0.474559 px
Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112)
 Iteration 1: RMSD = 11.2267 px
 Iteration 2: RMSD = 4.39044 px
 Iteration 3: RMSD = 0.604494 px
 Iteration 4: RMSD = 0.404841 px
Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112)
 Iteration 1: RMSD = 3.09543 px
 Iteration 2: RMSD = 0.667073 px
 Iteration 3: RMSD = 0.250155 px
Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854)
 Iteration 1: RMSD = 13.416 px
 Iteration 2: RMSD = 5.86998 px
 Iteration 3: RMSD = 0.972308 px
 Iteration 4: RMSD = 0.42697 px
Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854)
 Iteration 1: RMSD = 11.2506 px
 Iteration 2: RMSD = 12.2588 px
 Iteration 3: RMSD = 7.02891 px
 Iteration 4: RMSD = 1.65252 px
 Iteration 5: RMSD = 1.24539 px
Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854)
 Iteration 1: RMSD = 9.72045 px
 Iteration 2: RMSD = 7.92404 px
 Iteration 3: RMSD = 9.39133 px
 Iteration 4: RMSD = 6.48282 px
 Iteration 5: RMSD = 6.43874 px
Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854)
 Iteration 1: RMSD = 10.742 px
 Iteration 2: RMSD = 1.04174 px
 Iteration 3: RMSD = 0.432767 px
Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854)
 Iteration 1: RMSD = 2.16493 px
 Iteration 2: RMSD = 0.766729 px
 Iteration 3: RMSD = 0.231645 px
Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596)
 Iteration 1: RMSD = 11.3097 px
 Iteration 2: RMSD = 6.62308 px
 Iteration 3: RMSD = 4.81651 px
 Iteration 4: RMSD = 1.05274 px
 Iteration 5: RMSD = 0.515113 px
Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596)
 Iteration 1: RMSD = 9.64513 px
 Iteration 2: RMSD = 7.19919 px
 Iteration 3: RMSD = 5.2017 px
 Iteration 4: RMSD = 5.08027 px
 Iteration 5: RMSD = 5.09633 px
Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596)
 Iteration 1: RMSD = 15.2499 px
 Iteration 2: RMSD = 6.25637 px
 Iteration 3: RMSD = 5.29935 px
 Iteration 4: RMSD = 4.99443 px
 Iteration 5: RMSD = 4.99056 px
Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596)
 Iteration 1: RMSD = 7.67677 px
 Iteration 2: RMSD = 2.8946 px
 Iteration 3: RMSD = 0.369598 px
Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596)
 Iteration 1: RMSD = 3.1072 px
 Iteration 2: RMSD = 0.405208 px
Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338)
 Iteration 1: RMSD = 10.1231 px
 Iteration 2: RMSD = 5.60001 px
 Iteration 3: RMSD = 5.31662 px
 Iteration 4: RMSD = 5.03286 px
 Iteration 5: RMSD = 1.55586 px
Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338)
 Iteration 1: RMSD = 9.95189 px
 Iteration 2: RMSD = 5.51707 px
 Iteration 3: RMSD = 5.41898 px
 Iteration 4: RMSD = 5.3344 px
 Iteration 5: RMSD = 5.17729 px
Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338)
 Iteration 1: RMSD = 31.5736 px
 Iteration 2: RMSD = 1.00186 px
 Iteration 3: RMSD = 0.71783 px
 Iteration 4: RMSD = 0.284386 px
Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338)
 Iteration 1: RMSD = 8.53151 px
 Iteration 2: RMSD = 0.945869 px
 Iteration 3: RMSD = 0.335821 px
Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338)
 Iteration 1: RMSD = 2.00074 px
 Iteration 2: RMSD = 0.512188 px
 Iteration 3: RMSD = 0.136205 px

Polynomial fit RMSD: X = 7.685 px Y = 15.1334 px
The polynomial motion model did not explain the observation very well.
Local correction is disabled for this micrograph.
Summing frames after dose weighting: ................................................................................ done
Written aligned and dose-weighted sum to MotionCorr/job002/249_002.mrc