Working on 248-95_003.tif with 10 thread(s).

Movie size: X = 3838 Y = 3710 N = 80
Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80
Frame grouping: n_frames = 80, requested group size = 1
 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 
interpolate_shifts = 0

In unaligned sum, Mean = 27.0569 Std = 5.64333 Hotpixel threshold = 60.9169
Detected 281 hot pixels to be corrected.
Fixed hot pixels.

Global alignment:
 Iteration 1: RMSD = 6.29226 px
 Iteration 2: RMSD = 0.158044 px

Local alignments:
Patches: X = 5 Y = 5
Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370)
 Iteration 1: RMSD = 2.37524 px
 Iteration 2: RMSD = 0.520131 px
 Iteration 3: RMSD = 0.154773 px
Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370)
 Iteration 1: RMSD = 4.52569 px
 Iteration 2: RMSD = 0.878903 px
 Iteration 3: RMSD = 0.30103 px
Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370)
 Iteration 1: RMSD = 3.71308 px
 Iteration 2: RMSD = 1.21376 px
 Iteration 3: RMSD = 0.639102 px
 Iteration 4: RMSD = 0.285439 px
Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370)
 Iteration 1: RMSD = 13.1763 px
 Iteration 2: RMSD = 2.91129 px
 Iteration 3: RMSD = 0.872187 px
 Iteration 4: RMSD = 0.60504 px
 Iteration 5: RMSD = 0.29317 px
Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370)
 Iteration 1: RMSD = 12.6948 px
 Iteration 2: RMSD = 20.2537 px
 Iteration 3: RMSD = 23.2645 px
 Iteration 4: RMSD = 23.3196 px
 Iteration 5: RMSD = 22.2882 px
Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112)
 Iteration 1: RMSD = 3.44658 px
 Iteration 2: RMSD = 0.58013 px
 Iteration 3: RMSD = 0.216838 px
Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112)
 Iteration 1: RMSD = 4.18761 px
 Iteration 2: RMSD = 2.3983 px
 Iteration 3: RMSD = 0.81142 px
 Iteration 4: RMSD = 0.596999 px
 Iteration 5: RMSD = 0.335376 px
Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112)
 Iteration 1: RMSD = 8.948 px
 Iteration 2: RMSD = 2.16763 px
 Iteration 3: RMSD = 1.04405 px
 Iteration 4: RMSD = 0.490705 px
Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112)
 Iteration 1: RMSD = 13.2201 px
 Iteration 2: RMSD = 6.85866 px
 Iteration 3: RMSD = 5.54975 px
 Iteration 4: RMSD = 3.52228 px
 Iteration 5: RMSD = 5.01807 px
Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112)
 Iteration 1: RMSD = 8.96523 px
 Iteration 2: RMSD = 1.13024 px
 Iteration 3: RMSD = 0.374669 px
Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854)
 Iteration 1: RMSD = 2.14179 px
 Iteration 2: RMSD = 0.692002 px
 Iteration 3: RMSD = 0.133933 px
Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854)
 Iteration 1: RMSD = 7.15492 px
 Iteration 2: RMSD = 1.13829 px
 Iteration 3: RMSD = 0.42637 px
Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854)
 Iteration 1: RMSD = 4.2905 px
 Iteration 2: RMSD = 1.44441 px
 Iteration 3: RMSD = 0.421878 px
Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854)
 Iteration 1: RMSD = 7.17092 px
 Iteration 2: RMSD = 6.57699 px
 Iteration 3: RMSD = 5.72278 px
 Iteration 4: RMSD = 5.54003 px
 Iteration 5: RMSD = 5.47723 px
Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854)
 Iteration 1: RMSD = 5.80712 px
 Iteration 2: RMSD = 2.26417 px
 Iteration 3: RMSD = 1.74716 px
 Iteration 4: RMSD = 1.68851 px
 Iteration 5: RMSD = 0.828689 px
Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596)
 Iteration 1: RMSD = 3.73901 px
 Iteration 2: RMSD = 0.534411 px
 Iteration 3: RMSD = 0.13476 px
Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596)
 Iteration 1: RMSD = 3.65519 px
 Iteration 2: RMSD = 0.53118 px
 Iteration 3: RMSD = 0.148669 px
Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596)
 Iteration 1: RMSD = 5.48378 px
 Iteration 2: RMSD = 0.881284 px
 Iteration 3: RMSD = 0.291152 px
Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596)
 Iteration 1: RMSD = 6.44766 px
 Iteration 2: RMSD = 0.810067 px
 Iteration 3: RMSD = 0.453551 px
Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596)
 Iteration 1: RMSD = 3.67248 px
 Iteration 2: RMSD = 0.85626 px
 Iteration 3: RMSD = 0.597148 px
 Iteration 4: RMSD = 0.276836 px
Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338)
 Iteration 1: RMSD = 4.1549 px
 Iteration 2: RMSD = 0.843261 px
 Iteration 3: RMSD = 0.232951 px
Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338)
 Iteration 1: RMSD = 3.67352 px
 Iteration 2: RMSD = 0.452657 px
Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338)
 Iteration 1: RMSD = 3.22243 px
 Iteration 2: RMSD = 0.640808 px
 Iteration 3: RMSD = 0.288812 px
Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338)
 Iteration 1: RMSD = 4.35264 px
 Iteration 2: RMSD = 1.22129 px
 Iteration 3: RMSD = 0.803618 px
 Iteration 4: RMSD = 0.697243 px
 Iteration 5: RMSD = 0.721683 px
Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338)
 Iteration 1: RMSD = 5.19892 px
 Iteration 2: RMSD = 1.07795 px
 Iteration 3: RMSD = 0.467298 px

Polynomial fit RMSD: X = 3.34582 px Y = 2.84325 px
Summing frames after dose weighting: ................................................................................ done
Written aligned and dose-weighted sum to MotionCorr/job002/248-95_003.mrc