Working on 248-80_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 27.3747 Std = 5.74989 Hotpixel threshold = 61.874 Detected 260 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 3.7336 px Iteration 2: RMSD = 0.372293 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 2.4877 px Iteration 2: RMSD = 0.685266 px Iteration 3: RMSD = 0.191466 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 5.02826 px Iteration 2: RMSD = 3.49949 px Iteration 3: RMSD = 0.766684 px Iteration 4: RMSD = 0.426174 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 12.0831 px Iteration 2: RMSD = 6.82084 px Iteration 3: RMSD = 1.03314 px Iteration 4: RMSD = 0.932903 px Iteration 5: RMSD = 0.387508 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 14.9909 px Iteration 2: RMSD = 9.56984 px Iteration 3: RMSD = 8.97059 px Iteration 4: RMSD = 10.8131 px Iteration 5: RMSD = 8.65028 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 15.3593 px Iteration 2: RMSD = 6.73236 px Iteration 3: RMSD = 6.55703 px Iteration 4: RMSD = 4.64916 px Iteration 5: RMSD = 4.62131 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 3.11218 px Iteration 2: RMSD = 0.86902 px Iteration 3: RMSD = 0.149485 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 2.23463 px Iteration 2: RMSD = 0.650526 px Iteration 3: RMSD = 0.331122 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 8.20837 px Iteration 2: RMSD = 3.55452 px Iteration 3: RMSD = 4.42069 px Iteration 4: RMSD = 4.48946 px Iteration 5: RMSD = 4.40774 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 43.0607 px Iteration 2: RMSD = 8.60216 px Iteration 3: RMSD = 7.19334 px Iteration 4: RMSD = 6.17284 px Iteration 5: RMSD = 6.21342 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 14.8724 px Iteration 2: RMSD = 6.73621 px Iteration 3: RMSD = 6.6119 px Iteration 4: RMSD = 7.64715 px Iteration 5: RMSD = 7.66119 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 5.06059 px Iteration 2: RMSD = 0.56005 px Iteration 3: RMSD = 0.249455 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 4.47151 px Iteration 2: RMSD = 0.607457 px Iteration 3: RMSD = 0.281249 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 4.59634 px Iteration 2: RMSD = 1.03474 px Iteration 3: RMSD = 0.532923 px Iteration 4: RMSD = 0.399303 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 14.9177 px Iteration 2: RMSD = 9.67135 px Iteration 3: RMSD = 7.62198 px Iteration 4: RMSD = 3.28472 px Iteration 5: RMSD = 3.31089 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 13.2526 px Iteration 2: RMSD = 3.96309 px Iteration 3: RMSD = 3.12253 px Iteration 4: RMSD = 2.97507 px Iteration 5: RMSD = 3.00402 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 3.25925 px Iteration 2: RMSD = 0.435481 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 4.72711 px Iteration 2: RMSD = 0.563498 px Iteration 3: RMSD = 0.240328 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 2.89087 px Iteration 2: RMSD = 1.36862 px Iteration 3: RMSD = 0.542236 px Iteration 4: RMSD = 0.308915 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 2.97416 px Iteration 2: RMSD = 1.10491 px Iteration 3: RMSD = 0.49113 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 13.9301 px Iteration 2: RMSD = 12.9372 px Iteration 3: RMSD = 11.4541 px Iteration 4: RMSD = 10.0887 px Iteration 5: RMSD = 9.64293 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 4.98497 px Iteration 2: RMSD = 0.288287 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 3.60385 px Iteration 2: RMSD = 0.38495 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 3.99627 px Iteration 2: RMSD = 0.538751 px Iteration 3: RMSD = 0.180173 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 4.53916 px Iteration 2: RMSD = 1.02559 px Iteration 3: RMSD = 0.372142 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 4.83306 px Iteration 2: RMSD = 3.00145 px Iteration 3: RMSD = 2.89486 px Iteration 4: RMSD = 5.05505 px Iteration 5: RMSD = 0.358542 px Polynomial fit RMSD: X = 3.02886 px Y = 2.91832 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/248-80_003.mrc