Working on 248-79_001.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 27.8709 Std = 5.70109 Hotpixel threshold = 62.0774 Detected 279 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 6.46708 px Iteration 2: RMSD = 0.624008 px Iteration 3: RMSD = 0.0842156 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 2.69455 px Iteration 2: RMSD = 0.377457 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 2.69017 px Iteration 2: RMSD = 0.533227 px Iteration 3: RMSD = 0.156876 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 4.16777 px Iteration 2: RMSD = 0.501817 px Iteration 3: RMSD = 0.18899 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 5.34796 px Iteration 2: RMSD = 0.578859 px Iteration 3: RMSD = 0.278158 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 4.13071 px Iteration 2: RMSD = 0.411292 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 6.20308 px Iteration 2: RMSD = 1.41728 px Iteration 3: RMSD = 1.07689 px Iteration 4: RMSD = 1.04762 px Iteration 5: RMSD = 1.04266 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 4.33913 px Iteration 2: RMSD = 2.42516 px Iteration 3: RMSD = 0.443103 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 7.94147 px Iteration 2: RMSD = 1.28936 px Iteration 3: RMSD = 0.530009 px Iteration 4: RMSD = 0.225215 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 6.73242 px Iteration 2: RMSD = 1.70846 px Iteration 3: RMSD = 0.523571 px Iteration 4: RMSD = 0.543651 px Iteration 5: RMSD = 0.395475 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 3.62992 px Iteration 2: RMSD = 0.658699 px Iteration 3: RMSD = 0.341015 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 6.2005 px Iteration 2: RMSD = 2.87678 px Iteration 3: RMSD = 1.46413 px Iteration 4: RMSD = 0.498433 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 10.3898 px Iteration 2: RMSD = 5.83882 px Iteration 3: RMSD = 1.68376 px Iteration 4: RMSD = 1.35027 px Iteration 5: RMSD = 0.615418 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 11.271 px Iteration 2: RMSD = 5.55353 px Iteration 3: RMSD = 3.41004 px Iteration 4: RMSD = 3.32117 px Iteration 5: RMSD = 1.2953 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 11.9035 px Iteration 2: RMSD = 7.83046 px Iteration 3: RMSD = 4.91341 px Iteration 4: RMSD = 4.95247 px Iteration 5: RMSD = 4.97148 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 5.56081 px Iteration 2: RMSD = 1.00746 px Iteration 3: RMSD = 0.376049 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 39.9038 px Iteration 2: RMSD = 49.2952 px Iteration 3: RMSD = 4.99556 px Iteration 4: RMSD = 4.96203 px Iteration 5: RMSD = 4.98037 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 17.9199 px Iteration 2: RMSD = 18.1114 px Iteration 3: RMSD = 15.947 px Iteration 4: RMSD = 18.6409 px Iteration 5: RMSD = 15.2689 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 16.7913 px Iteration 2: RMSD = 12.2427 px Iteration 3: RMSD = 10.4374 px Iteration 4: RMSD = 12.0548 px Iteration 5: RMSD = 13.0667 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 11.5308 px Iteration 2: RMSD = 7.15197 px Iteration 3: RMSD = 7.81808 px Iteration 4: RMSD = 5.45406 px Iteration 5: RMSD = 5.2902 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 7.31962 px Iteration 2: RMSD = 1.35953 px Iteration 3: RMSD = 0.81863 px Iteration 4: RMSD = 0.771958 px Iteration 5: RMSD = 0.307109 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 13.086 px Iteration 2: RMSD = 7.42338 px Iteration 3: RMSD = 1.54957 px Iteration 4: RMSD = 0.821477 px Iteration 5: RMSD = 0.312312 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 18.8868 px Iteration 2: RMSD = 1.6164 px Iteration 3: RMSD = 0.705028 px Iteration 4: RMSD = 0.435675 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 18.8158 px Iteration 2: RMSD = 10.7632 px Iteration 3: RMSD = 4.14927 px Iteration 4: RMSD = 4.28773 px Iteration 5: RMSD = 4.04592 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 15.8139 px Iteration 2: RMSD = 4.48965 px Iteration 3: RMSD = 2.41073 px Iteration 4: RMSD = 2.19285 px Iteration 5: RMSD = 1.06399 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 14.1293 px Iteration 2: RMSD = 9.97652 px Iteration 3: RMSD = 8.6426 px Iteration 4: RMSD = 8.63228 px Iteration 5: RMSD = 6.40765 px Polynomial fit RMSD: X = 5.10769 px Y = 3.51414 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/248-79_001.mrc