Working on 239-101_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 28.9285 Std = 5.6517 Hotpixel threshold = 62.8387 Detected 265 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 5.38363 px Iteration 2: RMSD = 0.365508 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 14.0083 px Iteration 2: RMSD = 12.4659 px Iteration 3: RMSD = 10.9946 px Iteration 4: RMSD = 7.13092 px Iteration 5: RMSD = 6.29775 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 15.6056 px Iteration 2: RMSD = 12.875 px Iteration 3: RMSD = 10.7266 px Iteration 4: RMSD = 11.9382 px Iteration 5: RMSD = 13.3692 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 12.7719 px Iteration 2: RMSD = 11.7748 px Iteration 3: RMSD = 10.6356 px Iteration 4: RMSD = 10.905 px Iteration 5: RMSD = 12.1976 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 8.47491 px Iteration 2: RMSD = 7.46222 px Iteration 3: RMSD = 8.18336 px Iteration 4: RMSD = 8.95972 px Iteration 5: RMSD = 8.92437 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 5.38238 px Iteration 2: RMSD = 0.933797 px Iteration 3: RMSD = 0.366782 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 29.3439 px Iteration 2: RMSD = 21.6104 px Iteration 3: RMSD = 22.0276 px Iteration 4: RMSD = 22.776 px Iteration 5: RMSD = 21.6286 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 23.6618 px Iteration 2: RMSD = 23.5027 px Iteration 3: RMSD = 20.9759 px Iteration 4: RMSD = 20.8494 px Iteration 5: RMSD = 56.2333 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 23.3348 px Iteration 2: RMSD = 19.0287 px Iteration 3: RMSD = 22.0319 px Iteration 4: RMSD = 18.3002 px Iteration 5: RMSD = 18.0575 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 7.70818 px Iteration 2: RMSD = 5.33256 px Iteration 3: RMSD = 5.80296 px Iteration 4: RMSD = 5.29112 px Iteration 5: RMSD = 6.05114 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 5.12861 px Iteration 2: RMSD = 1.21104 px Iteration 3: RMSD = 0.838333 px Iteration 4: RMSD = 0.390895 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 24.2866 px Iteration 2: RMSD = 21.8963 px Iteration 3: RMSD = 19.7386 px Iteration 4: RMSD = 20.6838 px Iteration 5: RMSD = 20.6518 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 20.5223 px Iteration 2: RMSD = 14.603 px Iteration 3: RMSD = 13.9532 px Iteration 4: RMSD = 14.9855 px Iteration 5: RMSD = 14.5718 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 17.671 px Iteration 2: RMSD = 12.7348 px Iteration 3: RMSD = 11.4967 px Iteration 4: RMSD = 10.84 px Iteration 5: RMSD = 9.35655 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 13.8856 px Iteration 2: RMSD = 10.2351 px Iteration 3: RMSD = 6.95167 px Iteration 4: RMSD = 6.45875 px Iteration 5: RMSD = 3.52575 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 10.4968 px Iteration 2: RMSD = 0.913349 px Iteration 3: RMSD = 0.390242 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 28.3377 px Iteration 2: RMSD = 23.1908 px Iteration 3: RMSD = 17.8521 px Iteration 4: RMSD = 21.4079 px Iteration 5: RMSD = 19.4282 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 17.7066 px Iteration 2: RMSD = 20.9805 px Iteration 3: RMSD = 19.8329 px Iteration 4: RMSD = 19.4043 px Iteration 5: RMSD = 20.064 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 18.2135 px Iteration 2: RMSD = 13.9795 px Iteration 3: RMSD = 13.755 px Iteration 4: RMSD = 14.6522 px Iteration 5: RMSD = 13.9803 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 6.1297 px Iteration 2: RMSD = 1.73454 px Iteration 3: RMSD = 1.36795 px Iteration 4: RMSD = 1.20434 px Iteration 5: RMSD = 2.59493 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 5.47423 px Iteration 2: RMSD = 2.11404 px Iteration 3: RMSD = 1.35186 px Iteration 4: RMSD = 1.01704 px Iteration 5: RMSD = 0.573872 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 13.3201 px Iteration 2: RMSD = 14.3914 px Iteration 3: RMSD = 13.0799 px Iteration 4: RMSD = 12.5548 px Iteration 5: RMSD = 13.7941 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 11.6144 px Iteration 2: RMSD = 9.32368 px Iteration 3: RMSD = 7.85986 px Iteration 4: RMSD = 6.50634 px Iteration 5: RMSD = 6.52531 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 11.7745 px Iteration 2: RMSD = 7.15048 px Iteration 3: RMSD = 7.02932 px Iteration 4: RMSD = 5.19301 px Iteration 5: RMSD = 2.1501 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 8.09378 px Iteration 2: RMSD = 1.11382 px Iteration 3: RMSD = 2.22358 px Iteration 4: RMSD = 2.21676 px Iteration 5: RMSD = 2.19082 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 3.69017 px Iteration 2: RMSD = 0.803277 px Iteration 3: RMSD = 0.24072 px Polynomial fit RMSD: X = 3.00006 px Y = 4.23281 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/239-101_002.mrc