Working on 199-61_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 29.1795 Std = 5.80105 Hotpixel threshold = 63.9858 Detected 249 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 4.50115 px Iteration 2: RMSD = 0.453942 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 7.36899 px Iteration 2: RMSD = 2.1752 px Iteration 3: RMSD = 0.399255 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 3.24114 px Iteration 2: RMSD = 1.49984 px Iteration 3: RMSD = 1.0802 px Iteration 4: RMSD = 1.0871 px Iteration 5: RMSD = 1.17596 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 10.8018 px Iteration 2: RMSD = 7.18903 px Iteration 3: RMSD = 3.07734 px Iteration 4: RMSD = 1.73993 px Iteration 5: RMSD = 1.5608 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 9.36318 px Iteration 2: RMSD = 5.65162 px Iteration 3: RMSD = 4.45706 px Iteration 4: RMSD = 2.18422 px Iteration 5: RMSD = 2.31217 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 6.39776 px Iteration 2: RMSD = 2.04831 px Iteration 3: RMSD = 1.06527 px Iteration 4: RMSD = 1.14658 px Iteration 5: RMSD = 0.579131 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 4.80972 px Iteration 2: RMSD = 0.6811 px Iteration 3: RMSD = 0.300891 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 57.5752 px Iteration 2: RMSD = 9.33333 px Iteration 3: RMSD = 5.58493 px Iteration 4: RMSD = 0.999326 px Iteration 5: RMSD = 0.576663 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 14.2409 px Iteration 2: RMSD = 8.6206 px Iteration 3: RMSD = 7.93761 px Iteration 4: RMSD = 2.51651 px Iteration 5: RMSD = 0.966685 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 12.7419 px Iteration 2: RMSD = 10.9095 px Iteration 3: RMSD = 7.50335 px Iteration 4: RMSD = 0.917287 px Iteration 5: RMSD = 0.641176 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 8.75239 px Iteration 2: RMSD = 4.77695 px Iteration 3: RMSD = 4.75547 px Iteration 4: RMSD = 4.78303 px Iteration 5: RMSD = 4.82903 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 4.99444 px Iteration 2: RMSD = 0.895711 px Iteration 3: RMSD = 0.329404 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 5.75594 px Iteration 2: RMSD = 1.15454 px Iteration 3: RMSD = 0.592593 px Iteration 4: RMSD = 0.164773 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 12.8327 px Iteration 2: RMSD = 6.91541 px Iteration 3: RMSD = 11.6966 px Iteration 4: RMSD = 8.24382 px Iteration 5: RMSD = 0.655139 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 8.95899 px Iteration 2: RMSD = 5.81491 px Iteration 3: RMSD = 4.83465 px Iteration 4: RMSD = 4.84183 px Iteration 5: RMSD = 4.73881 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 11.4589 px Iteration 2: RMSD = 1.87904 px Iteration 3: RMSD = 5.65682 px Iteration 4: RMSD = 0.546727 px Iteration 5: RMSD = 0.260364 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 5.42672 px Iteration 2: RMSD = 0.406753 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 6.89177 px Iteration 2: RMSD = 1.25208 px Iteration 3: RMSD = 0.471012 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 12.226 px Iteration 2: RMSD = 6.28032 px Iteration 3: RMSD = 4.74338 px Iteration 4: RMSD = 4.44197 px Iteration 5: RMSD = 4.41942 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 12.2083 px Iteration 2: RMSD = 9.43619 px Iteration 3: RMSD = 9.66723 px Iteration 4: RMSD = 9.63427 px Iteration 5: RMSD = 9.70415 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 11.2045 px Iteration 2: RMSD = 8.08143 px Iteration 3: RMSD = 7.39753 px Iteration 4: RMSD = 6.84646 px Iteration 5: RMSD = 5.57597 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 3.00488 px Iteration 2: RMSD = 0.297817 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 4.82821 px Iteration 2: RMSD = 0.914513 px Iteration 3: RMSD = 0.394425 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 9.32143 px Iteration 2: RMSD = 5.62809 px Iteration 3: RMSD = 5.47025 px Iteration 4: RMSD = 5.50705 px Iteration 5: RMSD = 5.46838 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 11.1061 px Iteration 2: RMSD = 5.84473 px Iteration 3: RMSD = 2.12262 px Iteration 4: RMSD = 2.00035 px Iteration 5: RMSD = 2.02723 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 10.8151 px Iteration 2: RMSD = 7.78572 px Iteration 3: RMSD = 2.53978 px Iteration 4: RMSD = 2.28008 px Iteration 5: RMSD = 4.7175 px Polynomial fit RMSD: X = 3.75002 px Y = 2.26487 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/199-61_003.mrc