Working on 199-50_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 29.1931 Std = 5.63665 Hotpixel threshold = 63.013 Detected 255 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 6.81057 px Iteration 2: RMSD = 1.20665 px Iteration 3: RMSD = 0.653722 px Iteration 4: RMSD = 0.310224 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 7.66861 px Iteration 2: RMSD = 1.63897 px Iteration 3: RMSD = 0.930017 px Iteration 4: RMSD = 0.653656 px Iteration 5: RMSD = 0.262456 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 8.11903 px Iteration 2: RMSD = 4.36195 px Iteration 3: RMSD = 3.4372 px Iteration 4: RMSD = 3.97107 px Iteration 5: RMSD = 4.16183 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 34.5764 px Iteration 2: RMSD = 35.4012 px Iteration 3: RMSD = 12.1654 px Iteration 4: RMSD = 8.98731 px Iteration 5: RMSD = 10.6825 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 12.5438 px Iteration 2: RMSD = 10.6321 px Iteration 3: RMSD = 9.4107 px Iteration 4: RMSD = 8.08629 px Iteration 5: RMSD = 5.61282 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 7.79331 px Iteration 2: RMSD = 5.23733 px Iteration 3: RMSD = 5.68423 px Iteration 4: RMSD = 7.76442 px Iteration 5: RMSD = 7.82538 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 15.8168 px Iteration 2: RMSD = 12.0847 px Iteration 3: RMSD = 11.2155 px Iteration 4: RMSD = 9.38837 px Iteration 5: RMSD = 8.22992 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 4.71422 px Iteration 2: RMSD = 2.65896 px Iteration 3: RMSD = 3.74583 px Iteration 4: RMSD = 3.59272 px Iteration 5: RMSD = 3.51454 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 22.5959 px Iteration 2: RMSD = 43.0225 px Iteration 3: RMSD = 45.8464 px Iteration 4: RMSD = 45.1879 px Iteration 5: RMSD = 44.9975 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 18.3205 px Iteration 2: RMSD = 9.98818 px Iteration 3: RMSD = 9.90604 px Iteration 4: RMSD = 7.44953 px Iteration 5: RMSD = 3.2397 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 16.941 px Iteration 2: RMSD = 11.9649 px Iteration 3: RMSD = 12.6117 px Iteration 4: RMSD = 13.4956 px Iteration 5: RMSD = 12.6732 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 50.0556 px Iteration 2: RMSD = 14.4387 px Iteration 3: RMSD = 13.2563 px Iteration 4: RMSD = 11.7617 px Iteration 5: RMSD = 11.6202 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 21.2023 px Iteration 2: RMSD = 16.0063 px Iteration 3: RMSD = 14.1198 px Iteration 4: RMSD = 11.6966 px Iteration 5: RMSD = 10.3147 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 22.8545 px Iteration 2: RMSD = 18.1376 px Iteration 3: RMSD = 13.3198 px Iteration 4: RMSD = 12.2684 px Iteration 5: RMSD = 10.9134 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 26.6949 px Iteration 2: RMSD = 23.6108 px Iteration 3: RMSD = 22.2703 px Iteration 4: RMSD = 21.803 px Iteration 5: RMSD = 20.2948 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 17.3856 px Iteration 2: RMSD = 6.6471 px Iteration 3: RMSD = 3.48965 px Iteration 4: RMSD = 0.839486 px Iteration 5: RMSD = 0.166019 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 17.2094 px Iteration 2: RMSD = 11.7634 px Iteration 3: RMSD = 15.8331 px Iteration 4: RMSD = 14.3453 px Iteration 5: RMSD = 15.4088 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 18.9167 px Iteration 2: RMSD = 15.503 px Iteration 3: RMSD = 15.8811 px Iteration 4: RMSD = 29.8313 px Iteration 5: RMSD = 30.1435 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 20.3873 px Iteration 2: RMSD = 7.89337 px Iteration 3: RMSD = 7.52715 px Iteration 4: RMSD = 7.61156 px Iteration 5: RMSD = 7.67599 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 15.6731 px Iteration 2: RMSD = 6.05431 px Iteration 3: RMSD = 2.69149 px Iteration 4: RMSD = 0.504524 px Iteration 5: RMSD = 0.28711 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 7.13776 px Iteration 2: RMSD = 2.59481 px Iteration 3: RMSD = 2.42903 px Iteration 4: RMSD = 2.27729 px Iteration 5: RMSD = 2.13335 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 10.297 px Iteration 2: RMSD = 8.53849 px Iteration 3: RMSD = 6.93801 px Iteration 4: RMSD = 1.96153 px Iteration 5: RMSD = 1.65579 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 20.2004 px Iteration 2: RMSD = 13.0708 px Iteration 3: RMSD = 9.17979 px Iteration 4: RMSD = 6.89522 px Iteration 5: RMSD = 6.81957 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 16.9227 px Iteration 2: RMSD = 7.8664 px Iteration 3: RMSD = 5.78883 px Iteration 4: RMSD = 5.41578 px Iteration 5: RMSD = 5.4106 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 22.0423 px Iteration 2: RMSD = 7.51635 px Iteration 3: RMSD = 6.09621 px Iteration 4: RMSD = 6.42962 px Iteration 5: RMSD = 6.39519 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 10.9867 px Iteration 2: RMSD = 5.64131 px Iteration 3: RMSD = 0.81336 px Iteration 4: RMSD = 0.384003 px Polynomial fit RMSD: X = 2.86085 px Y = 4.06617 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/199-50_002.mrc