Working on 199-2_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 29.3386 Std = 5.65689 Hotpixel threshold = 63.2799 Detected 313 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 6.30969 px Iteration 2: RMSD = 0.862081 px Iteration 3: RMSD = 0.563794 px Iteration 4: RMSD = 0.211626 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 10.4412 px Iteration 2: RMSD = 9.11144 px Iteration 3: RMSD = 9.48074 px Iteration 4: RMSD = 11.7161 px Iteration 5: RMSD = 8.03544 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 18.3937 px Iteration 2: RMSD = 48.8992 px Iteration 3: RMSD = 47.3261 px Iteration 4: RMSD = 7.41693 px Iteration 5: RMSD = 5.30348 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 9.43533 px Iteration 2: RMSD = 10.1349 px Iteration 3: RMSD = 10.0749 px Iteration 4: RMSD = 10.0645 px Iteration 5: RMSD = 11.0962 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 37.5167 px Iteration 2: RMSD = 7.69163 px Iteration 3: RMSD = 5.59306 px Iteration 4: RMSD = 5.51767 px Iteration 5: RMSD = 5.42586 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 11.8016 px Iteration 2: RMSD = 1.73714 px Iteration 3: RMSD = 1.09107 px Iteration 4: RMSD = 0.475531 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 17.7118 px Iteration 2: RMSD = 8.36613 px Iteration 3: RMSD = 0.921209 px Iteration 4: RMSD = 0.37398 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 14.5546 px Iteration 2: RMSD = 13.1883 px Iteration 3: RMSD = 13.1167 px Iteration 4: RMSD = 10.4312 px Iteration 5: RMSD = 9.64671 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 14.8643 px Iteration 2: RMSD = 13.9104 px Iteration 3: RMSD = 13.6885 px Iteration 4: RMSD = 11.7456 px Iteration 5: RMSD = 11.7571 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 9.46418 px Iteration 2: RMSD = 3.25619 px Iteration 3: RMSD = 3.05881 px Iteration 4: RMSD = 2.92965 px Iteration 5: RMSD = 2.88789 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 8.7498 px Iteration 2: RMSD = 1.22466 px Iteration 3: RMSD = 0.615158 px Iteration 4: RMSD = 0.265003 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 15.5051 px Iteration 2: RMSD = 9.74197 px Iteration 3: RMSD = 7.51464 px Iteration 4: RMSD = 7.35641 px Iteration 5: RMSD = 8.92791 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 13.1879 px Iteration 2: RMSD = 8.63024 px Iteration 3: RMSD = 6.51522 px Iteration 4: RMSD = 4.6445 px Iteration 5: RMSD = 4.59044 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 11.4727 px Iteration 2: RMSD = 2.02136 px Iteration 3: RMSD = 0.589071 px Iteration 4: RMSD = 0.422055 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 17.5981 px Iteration 2: RMSD = 6.12902 px Iteration 3: RMSD = 2.89289 px Iteration 4: RMSD = 1.65139 px Iteration 5: RMSD = 0.830743 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 15.5096 px Iteration 2: RMSD = 1.87036 px Iteration 3: RMSD = 0.664844 px Iteration 4: RMSD = 0.283469 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 49.6644 px Iteration 2: RMSD = 20.4959 px Iteration 3: RMSD = 28.2449 px Iteration 4: RMSD = 28.0986 px Iteration 5: RMSD = 27.7086 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 18.5134 px Iteration 2: RMSD = 12.4717 px Iteration 3: RMSD = 9.7113 px Iteration 4: RMSD = 9.80799 px Iteration 5: RMSD = 9.71501 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 12.6297 px Iteration 2: RMSD = 8.37645 px Iteration 3: RMSD = 7.70092 px Iteration 4: RMSD = 7.62768 px Iteration 5: RMSD = 7.60751 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 15.0559 px Iteration 2: RMSD = 4.82308 px Iteration 3: RMSD = 4.50545 px Iteration 4: RMSD = 4.50837 px Iteration 5: RMSD = 0.35535 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 11.1101 px Iteration 2: RMSD = 1.37796 px Iteration 3: RMSD = 0.548986 px Iteration 4: RMSD = 0.412283 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 4.17443 px Iteration 2: RMSD = 2.12904 px Iteration 3: RMSD = 1.59822 px Iteration 4: RMSD = 0.584134 px Iteration 5: RMSD = 0.342059 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 13.8483 px Iteration 2: RMSD = 8.19599 px Iteration 3: RMSD = 8.12999 px Iteration 4: RMSD = 8.15158 px Iteration 5: RMSD = 8.09971 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 12.6113 px Iteration 2: RMSD = 5.35628 px Iteration 3: RMSD = 5.04116 px Iteration 4: RMSD = 5.088 px Iteration 5: RMSD = 5.03681 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 19.1764 px Iteration 2: RMSD = 4.53122 px Iteration 3: RMSD = 1.43449 px Iteration 4: RMSD = 0.235307 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 10.0432 px Iteration 2: RMSD = 0.839942 px Iteration 3: RMSD = 0.601556 px Iteration 4: RMSD = 0.140782 px Polynomial fit RMSD: X = 5.90331 px Y = 3.61639 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/199-2_002.mrc