Working on 165-86_001.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 28.8582 Std = 5.57988 Hotpixel threshold = 62.3375 Detected 275 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 11.8273 px Iteration 2: RMSD = 1.19074 px Iteration 3: RMSD = 0.615619 px Iteration 4: RMSD = 0.224378 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 3.97904 px Iteration 2: RMSD = 0.287636 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 5.9202 px Iteration 2: RMSD = 0.79651 px Iteration 3: RMSD = 0.52988 px Iteration 4: RMSD = 0.095749 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 6.39356 px Iteration 2: RMSD = 0.846948 px Iteration 3: RMSD = 0.231387 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 7.61557 px Iteration 2: RMSD = 1.31839 px Iteration 3: RMSD = 0.55985 px Iteration 4: RMSD = 0.198279 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 4.8828 px Iteration 2: RMSD = 0.698277 px Iteration 3: RMSD = 0.192531 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 4.40913 px Iteration 2: RMSD = 0.546932 px Iteration 3: RMSD = 0.180602 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 8.03185 px Iteration 2: RMSD = 3.73834 px Iteration 3: RMSD = 3.84112 px Iteration 4: RMSD = 3.77425 px Iteration 5: RMSD = 3.80941 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 4.56943 px Iteration 2: RMSD = 0.9809 px Iteration 3: RMSD = 0.249917 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 5.75623 px Iteration 2: RMSD = 1.78558 px Iteration 3: RMSD = 0.740047 px Iteration 4: RMSD = 0.178219 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 7.75577 px Iteration 2: RMSD = 4.98365 px Iteration 3: RMSD = 4.84154 px Iteration 4: RMSD = 5.45046 px Iteration 5: RMSD = 3.65398 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 51.3938 px Iteration 2: RMSD = 14.5537 px Iteration 3: RMSD = 10.5515 px Iteration 4: RMSD = 13.8598 px Iteration 5: RMSD = 8.23018 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 9.99747 px Iteration 2: RMSD = 5.68461 px Iteration 3: RMSD = 0.622322 px Iteration 4: RMSD = 0.16938 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 7.81455 px Iteration 2: RMSD = 6.90713 px Iteration 3: RMSD = 0.631154 px Iteration 4: RMSD = 0.162609 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 15.6169 px Iteration 2: RMSD = 9.68839 px Iteration 3: RMSD = 5.96609 px Iteration 4: RMSD = 4.80691 px Iteration 5: RMSD = 4.80491 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 8.98666 px Iteration 2: RMSD = 4.06403 px Iteration 3: RMSD = 1.77736 px Iteration 4: RMSD = 0.331503 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 18.1283 px Iteration 2: RMSD = 16.6338 px Iteration 3: RMSD = 11.6688 px Iteration 4: RMSD = 11.8036 px Iteration 5: RMSD = 13.1491 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 18.8517 px Iteration 2: RMSD = 54.1934 px Iteration 3: RMSD = 40.0672 px Iteration 4: RMSD = 39.6943 px Iteration 5: RMSD = 39.8937 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 23.9874 px Iteration 2: RMSD = 26.6672 px Iteration 3: RMSD = 26.7799 px Iteration 4: RMSD = 27.1378 px Iteration 5: RMSD = 27.581 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 21.624 px Iteration 2: RMSD = 5.75864 px Iteration 3: RMSD = 2.60476 px Iteration 4: RMSD = 2.47121 px Iteration 5: RMSD = 2.38063 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 15.5007 px Iteration 2: RMSD = 4.52836 px Iteration 3: RMSD = 1.98142 px Iteration 4: RMSD = 5.38919 px Iteration 5: RMSD = 2.01517 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 19.0615 px Iteration 2: RMSD = 8.47852 px Iteration 3: RMSD = 2.99727 px Iteration 4: RMSD = 0.959051 px Iteration 5: RMSD = 0.288023 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 26.755 px Iteration 2: RMSD = 8.95657 px Iteration 3: RMSD = 0.869114 px Iteration 4: RMSD = 0.278825 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 35.9129 px Iteration 2: RMSD = 41.2693 px Iteration 3: RMSD = 7.93531 px Iteration 4: RMSD = 9.70098 px Iteration 5: RMSD = 8.03758 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 42.7454 px Iteration 2: RMSD = 11.4614 px Iteration 3: RMSD = 7.04044 px Iteration 4: RMSD = 6.49266 px Iteration 5: RMSD = 2.86014 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 48.601 px Iteration 2: RMSD = 7.56124 px Iteration 3: RMSD = 5.40633 px Iteration 4: RMSD = 6.68084 px Iteration 5: RMSD = 7.39966 px Polynomial fit RMSD: X = 5.33638 px Y = 3.65898 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/165-86_001.mrc