Working on 165-62_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 28.6468 Std = 5.62972 Hotpixel threshold = 62.4251 Detected 239 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 1.83798 px Iteration 2: RMSD = 0.324857 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 2.89727 px Iteration 2: RMSD = 0.692604 px Iteration 3: RMSD = 0.430344 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 2.93507 px Iteration 2: RMSD = 1.1697 px Iteration 3: RMSD = 0.766927 px Iteration 4: RMSD = 0.401497 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 6.58509 px Iteration 2: RMSD = 6.54197 px Iteration 3: RMSD = 6.5468 px Iteration 4: RMSD = 6.39469 px Iteration 5: RMSD = 6.39961 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 8.69285 px Iteration 2: RMSD = 6.84563 px Iteration 3: RMSD = 5.53011 px Iteration 4: RMSD = 7.73303 px Iteration 5: RMSD = 7.5757 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 5.29512 px Iteration 2: RMSD = 4.30786 px Iteration 3: RMSD = 1.11911 px Iteration 4: RMSD = 0.730974 px Iteration 5: RMSD = 0.431967 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 3.30401 px Iteration 2: RMSD = 0.72474 px Iteration 3: RMSD = 0.326347 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 5.62847 px Iteration 2: RMSD = 0.981349 px Iteration 3: RMSD = 0.651258 px Iteration 4: RMSD = 0.192145 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 7.64532 px Iteration 2: RMSD = 7.78241 px Iteration 3: RMSD = 7.74001 px Iteration 4: RMSD = 6.33699 px Iteration 5: RMSD = 4.84499 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 12.8843 px Iteration 2: RMSD = 7.58689 px Iteration 3: RMSD = 7.40833 px Iteration 4: RMSD = 7.41589 px Iteration 5: RMSD = 6.32972 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 10.8879 px Iteration 2: RMSD = 10.1647 px Iteration 3: RMSD = 10.0437 px Iteration 4: RMSD = 9.92981 px Iteration 5: RMSD = 9.93047 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 3.74922 px Iteration 2: RMSD = 0.759482 px Iteration 3: RMSD = 0.358691 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 6.04674 px Iteration 2: RMSD = 1.4094 px Iteration 3: RMSD = 0.482791 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 7.70678 px Iteration 2: RMSD = 3.95724 px Iteration 3: RMSD = 3.5533 px Iteration 4: RMSD = 3.52746 px Iteration 5: RMSD = 8.88531 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 11.401 px Iteration 2: RMSD = 10.2805 px Iteration 3: RMSD = 7.53466 px Iteration 4: RMSD = 7.9626 px Iteration 5: RMSD = 6.12154 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 10.583 px Iteration 2: RMSD = 5.93408 px Iteration 3: RMSD = 3.43764 px Iteration 4: RMSD = 3.41652 px Iteration 5: RMSD = 2.85359 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 4.65388 px Iteration 2: RMSD = 0.481847 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 7.00245 px Iteration 2: RMSD = 1.73118 px Iteration 3: RMSD = 1.56207 px Iteration 4: RMSD = 0.802455 px Iteration 5: RMSD = 0.301432 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 8.18918 px Iteration 2: RMSD = 1.35916 px Iteration 3: RMSD = 0.941791 px Iteration 4: RMSD = 0.631492 px Iteration 5: RMSD = 3.97571 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 10.4074 px Iteration 2: RMSD = 0.987651 px Iteration 3: RMSD = 0.488371 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 3.23792 px Iteration 2: RMSD = 1.24923 px Iteration 3: RMSD = 0.248531 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 5.03043 px Iteration 2: RMSD = 0.510634 px Iteration 3: RMSD = 0.112226 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 6.87833 px Iteration 2: RMSD = 0.864417 px Iteration 3: RMSD = 0.350258 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 3.10701 px Iteration 2: RMSD = 0.970515 px Iteration 3: RMSD = 0.603039 px Iteration 4: RMSD = 0.367944 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 3.34118 px Iteration 2: RMSD = 1.14858 px Iteration 3: RMSD = 0.780369 px Iteration 4: RMSD = 0.720585 px Iteration 5: RMSD = 0.717624 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 8.09037 px Iteration 2: RMSD = 11.7496 px Iteration 3: RMSD = 11.8594 px Iteration 4: RMSD = 11.6498 px Iteration 5: RMSD = 10.3819 px Polynomial fit RMSD: X = 4.2905 px Y = 2.87839 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/165-62_003.mrc