Working on 165-16_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 28.1568 Std = 5.56451 Hotpixel threshold = 61.5439 Detected 312 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 5.72554 px Iteration 2: RMSD = 0.196582 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 10.384 px Iteration 2: RMSD = 4.95141 px Iteration 3: RMSD = 0.842556 px Iteration 4: RMSD = 0.474443 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 48.9143 px Iteration 2: RMSD = 35.0706 px Iteration 3: RMSD = 36.5246 px Iteration 4: RMSD = 37.0601 px Iteration 5: RMSD = 37.3258 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 5.42182 px Iteration 2: RMSD = 0.948734 px Iteration 3: RMSD = 0.216672 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 3.1198 px Iteration 2: RMSD = 0.741687 px Iteration 3: RMSD = 0.394977 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 3.44422 px Iteration 2: RMSD = 0.452641 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 8.25333 px Iteration 2: RMSD = 5.54961 px Iteration 3: RMSD = 0.62687 px Iteration 4: RMSD = 0.571448 px Iteration 5: RMSD = 0.565953 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 12.6699 px Iteration 2: RMSD = 4.68748 px Iteration 3: RMSD = 3.62369 px Iteration 4: RMSD = 3.62109 px Iteration 5: RMSD = 3.58037 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 7.46471 px Iteration 2: RMSD = 0.676862 px Iteration 3: RMSD = 0.253027 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 5.47023 px Iteration 2: RMSD = 0.661439 px Iteration 3: RMSD = 0.174223 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 2.99354 px Iteration 2: RMSD = 0.493371 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 16.0453 px Iteration 2: RMSD = 12.997 px Iteration 3: RMSD = 10.0678 px Iteration 4: RMSD = 12.3925 px Iteration 5: RMSD = 10.7816 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 6.8798 px Iteration 2: RMSD = 1.18182 px Iteration 3: RMSD = 0.466253 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 3.07768 px Iteration 2: RMSD = 1.35189 px Iteration 3: RMSD = 1.01557 px Iteration 4: RMSD = 0.582879 px Iteration 5: RMSD = 0.420906 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 7.59376 px Iteration 2: RMSD = 6.75209 px Iteration 3: RMSD = 6.42286 px Iteration 4: RMSD = 6.367 px Iteration 5: RMSD = 6.36299 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 4.76093 px Iteration 2: RMSD = 1.02256 px Iteration 3: RMSD = 0.403113 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 8.80222 px Iteration 2: RMSD = 1.91221 px Iteration 3: RMSD = 0.755389 px Iteration 4: RMSD = 0.539774 px Iteration 5: RMSD = 0.328752 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 9.88981 px Iteration 2: RMSD = 6.93333 px Iteration 3: RMSD = 6.75637 px Iteration 4: RMSD = 9.21398 px Iteration 5: RMSD = 8.76256 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 9.91095 px Iteration 2: RMSD = 8.59198 px Iteration 3: RMSD = 6.93416 px Iteration 4: RMSD = 6.92007 px Iteration 5: RMSD = 6.86378 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 3.74999 px Iteration 2: RMSD = 0.602699 px Iteration 3: RMSD = 0.213167 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 4.61951 px Iteration 2: RMSD = 0.57226 px Iteration 3: RMSD = 0.187737 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 9.77477 px Iteration 2: RMSD = 8.83146 px Iteration 3: RMSD = 5.56084 px Iteration 4: RMSD = 4.02893 px Iteration 5: RMSD = 4.03293 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 4.12176 px Iteration 2: RMSD = 2.6307 px Iteration 3: RMSD = 2.26363 px Iteration 4: RMSD = 0.470511 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 11.4251 px Iteration 2: RMSD = 7.33874 px Iteration 3: RMSD = 1.21075 px Iteration 4: RMSD = 2.83311 px Iteration 5: RMSD = 0.792933 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 5.46023 px Iteration 2: RMSD = 0.651727 px Iteration 3: RMSD = 0.280924 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 4.81743 px Iteration 2: RMSD = 0.578213 px Iteration 3: RMSD = 0.239578 px Polynomial fit RMSD: X = 3.29355 px Y = 2.79022 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/165-16_002.mrc