Working on 165-11_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 26.5381 Std = 6.18366 Hotpixel threshold = 63.6401 Detected 230 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 1.0547 px Iteration 2: RMSD = 0.0711775 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 5.68949 px Iteration 2: RMSD = 0.984196 px Iteration 3: RMSD = 0.375292 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 6.89832 px Iteration 2: RMSD = 3.67749 px Iteration 3: RMSD = 3.40209 px Iteration 4: RMSD = 0.549794 px Iteration 5: RMSD = 0.25487 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 1.88765 px Iteration 2: RMSD = 0.245632 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 7.43026 px Iteration 2: RMSD = 2.74008 px Iteration 3: RMSD = 0.692455 px Iteration 4: RMSD = 0.517192 px Iteration 5: RMSD = 0.15313 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 1.60168 px Iteration 2: RMSD = 0.788312 px Iteration 3: RMSD = 0.442316 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 2.68668 px Iteration 2: RMSD = 0.653496 px Iteration 3: RMSD = 0.262508 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 6.69019 px Iteration 2: RMSD = 4.40861 px Iteration 3: RMSD = 0.871221 px Iteration 4: RMSD = 0.432506 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 2.65328 px Iteration 2: RMSD = 0.81319 px Iteration 3: RMSD = 0.374017 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 3.50353 px Iteration 2: RMSD = 0.204845 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 4.06579 px Iteration 2: RMSD = 0.294063 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 4.14452 px Iteration 2: RMSD = 0.579234 px Iteration 3: RMSD = 0.202057 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 7.88707 px Iteration 2: RMSD = 3.98474 px Iteration 3: RMSD = 0.691701 px Iteration 4: RMSD = 0.308028 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 14.3613 px Iteration 2: RMSD = 9.38881 px Iteration 3: RMSD = 8.73537 px Iteration 4: RMSD = 8.59107 px Iteration 5: RMSD = 8.61217 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 20.6643 px Iteration 2: RMSD = 19.467 px Iteration 3: RMSD = 52.2797 px Iteration 4: RMSD = 52.6864 px Iteration 5: RMSD = 52.1695 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 7.51318 px Iteration 2: RMSD = 0.614899 px Iteration 3: RMSD = 0.200474 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 4.0368 px Iteration 2: RMSD = 0.702291 px Iteration 3: RMSD = 0.234137 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 7.23447 px Iteration 2: RMSD = 4.33136 px Iteration 3: RMSD = 0.50849 px Iteration 4: RMSD = 0.164121 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 5.75566 px Iteration 2: RMSD = 2.38169 px Iteration 3: RMSD = 1.81285 px Iteration 4: RMSD = 1.61622 px Iteration 5: RMSD = 1.58677 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 19.6215 px Iteration 2: RMSD = 22.5532 px Iteration 3: RMSD = 19.3263 px Iteration 4: RMSD = 19.406 px Iteration 5: RMSD = 50.5956 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 11.4335 px Iteration 2: RMSD = 4.23155 px Iteration 3: RMSD = 2.01059 px Iteration 4: RMSD = 4.20852 px Iteration 5: RMSD = 2.07513 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 6.38452 px Iteration 2: RMSD = 0.625553 px Iteration 3: RMSD = 0.340135 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 6.58836 px Iteration 2: RMSD = 3.2575 px Iteration 3: RMSD = 5.76457 px Iteration 4: RMSD = 5.76294 px Iteration 5: RMSD = 5.76414 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 8.06756 px Iteration 2: RMSD = 7.86458 px Iteration 3: RMSD = 6.5367 px Iteration 4: RMSD = 8.19697 px Iteration 5: RMSD = 8.2492 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 12.4497 px Iteration 2: RMSD = 2.00393 px Iteration 3: RMSD = 0.643898 px Iteration 4: RMSD = 0.200889 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 12.5996 px Iteration 2: RMSD = 8.33738 px Iteration 3: RMSD = 4.60037 px Iteration 4: RMSD = 3.80184 px Iteration 5: RMSD = 3.8557 px Polynomial fit RMSD: X = 3.05264 px Y = 3.89757 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/165-11_003.mrc