Working on 162-8_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 28.5847 Std = 5.57598 Hotpixel threshold = 62.0405 Detected 274 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 7.9585 px Iteration 2: RMSD = 0.726663 px Iteration 3: RMSD = 0.242771 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 16.546 px Iteration 2: RMSD = 11.9365 px Iteration 3: RMSD = 9.1055 px Iteration 4: RMSD = 9.85148 px Iteration 5: RMSD = 12.1029 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 16.5554 px Iteration 2: RMSD = 15.4187 px Iteration 3: RMSD = 13.731 px Iteration 4: RMSD = 12.5226 px Iteration 5: RMSD = 11.4966 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 4.70971 px Iteration 2: RMSD = 0.979568 px Iteration 3: RMSD = 0.604179 px Iteration 4: RMSD = 0.324318 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 7.57773 px Iteration 2: RMSD = 2.13418 px Iteration 3: RMSD = 0.681849 px Iteration 4: RMSD = 0.639024 px Iteration 5: RMSD = 0.228179 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 36.2849 px Iteration 2: RMSD = 3.91966 px Iteration 3: RMSD = 3.31426 px Iteration 4: RMSD = 3.33486 px Iteration 5: RMSD = 3.2458 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 14.0293 px Iteration 2: RMSD = 13.8034 px Iteration 3: RMSD = 12.8926 px Iteration 4: RMSD = 12.6288 px Iteration 5: RMSD = 11.3492 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 22.257 px Iteration 2: RMSD = 19.8067 px Iteration 3: RMSD = 18.7808 px Iteration 4: RMSD = 16.0081 px Iteration 5: RMSD = 13.597 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 44.7739 px Iteration 2: RMSD = 5.13854 px Iteration 3: RMSD = 1.82028 px Iteration 4: RMSD = 1.47948 px Iteration 5: RMSD = 1.02641 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 7.65427 px Iteration 2: RMSD = 7.40921 px Iteration 3: RMSD = 7.37034 px Iteration 4: RMSD = 6.77156 px Iteration 5: RMSD = 3.73652 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 26.867 px Iteration 2: RMSD = 25.1048 px Iteration 3: RMSD = 3.30891 px Iteration 4: RMSD = 2.11011 px Iteration 5: RMSD = 0.810403 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 17.4544 px Iteration 2: RMSD = 13.8668 px Iteration 3: RMSD = 11.6794 px Iteration 4: RMSD = 12.3742 px Iteration 5: RMSD = 12.0917 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 17.9954 px Iteration 2: RMSD = 14.6548 px Iteration 3: RMSD = 13.0603 px Iteration 4: RMSD = 11.1089 px Iteration 5: RMSD = 5.34643 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 18.2298 px Iteration 2: RMSD = 15.6977 px Iteration 3: RMSD = 17.1111 px Iteration 4: RMSD = 16.0382 px Iteration 5: RMSD = 15.0619 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 11.8712 px Iteration 2: RMSD = 7.10388 px Iteration 3: RMSD = 7.82518 px Iteration 4: RMSD = 4.1685 px Iteration 5: RMSD = 4.29159 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 9.02152 px Iteration 2: RMSD = 0.905254 px Iteration 3: RMSD = 0.578829 px Iteration 4: RMSD = 0.316024 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 21.1747 px Iteration 2: RMSD = 20.6426 px Iteration 3: RMSD = 15.586 px Iteration 4: RMSD = 16.7632 px Iteration 5: RMSD = 15.5976 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 10.5449 px Iteration 2: RMSD = 7.98064 px Iteration 3: RMSD = 8.08041 px Iteration 4: RMSD = 1.44951 px Iteration 5: RMSD = 0.208663 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 19.0172 px Iteration 2: RMSD = 12.1047 px Iteration 3: RMSD = 5.1076 px Iteration 4: RMSD = 3.07662 px Iteration 5: RMSD = 2.98248 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 15.9418 px Iteration 2: RMSD = 7.48502 px Iteration 3: RMSD = 7.16262 px Iteration 4: RMSD = 7.16275 px Iteration 5: RMSD = 7.10351 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 7.63627 px Iteration 2: RMSD = 4.03631 px Iteration 3: RMSD = 1.49084 px Iteration 4: RMSD = 0.521575 px Iteration 5: RMSD = 0.209686 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 6.38989 px Iteration 2: RMSD = 0.928138 px Iteration 3: RMSD = 0.311415 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 16.858 px Iteration 2: RMSD = 12.4943 px Iteration 3: RMSD = 9.3538 px Iteration 4: RMSD = 9.04096 px Iteration 5: RMSD = 7.42138 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 12.9191 px Iteration 2: RMSD = 9.43041 px Iteration 3: RMSD = 8.52611 px Iteration 4: RMSD = 10.0924 px Iteration 5: RMSD = 10.3025 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 13.055 px Iteration 2: RMSD = 1.75476 px Iteration 3: RMSD = 1.72998 px Iteration 4: RMSD = 2.38804 px Iteration 5: RMSD = 0.408501 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 6.09685 px Iteration 2: RMSD = 2.23132 px Iteration 3: RMSD = 2.15061 px Iteration 4: RMSD = 2.26373 px Iteration 5: RMSD = 2.3141 px Polynomial fit RMSD: X = 5.12333 px Y = 4.65473 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/162-8_002.mrc