Working on 139-37_001.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 29.3666 Std = 5.66668 Hotpixel threshold = 63.3667 Detected 295 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 7.25107 px Iteration 2: RMSD = 0.601937 px Iteration 3: RMSD = 0.129169 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 4.1983 px Iteration 2: RMSD = 0.749445 px Iteration 3: RMSD = 0.175678 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 6.68909 px Iteration 2: RMSD = 0.569831 px Iteration 3: RMSD = 0.240829 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 8.02492 px Iteration 2: RMSD = 0.560382 px Iteration 3: RMSD = 0.368781 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 8.17091 px Iteration 2: RMSD = 0.660769 px Iteration 3: RMSD = 0.23831 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 7.08952 px Iteration 2: RMSD = 0.541571 px Iteration 3: RMSD = 0.25795 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 6.27026 px Iteration 2: RMSD = 0.719997 px Iteration 3: RMSD = 0.504898 px Iteration 4: RMSD = 0.273413 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 4.44664 px Iteration 2: RMSD = 0.788352 px Iteration 3: RMSD = 0.611543 px Iteration 4: RMSD = 0.235539 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 2.84056 px Iteration 2: RMSD = 1.01627 px Iteration 3: RMSD = 0.466099 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 9.02406 px Iteration 2: RMSD = 3.33375 px Iteration 3: RMSD = 2.90301 px Iteration 4: RMSD = 2.7806 px Iteration 5: RMSD = 2.75856 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 7.01921 px Iteration 2: RMSD = 1.12407 px Iteration 3: RMSD = 0.675696 px Iteration 4: RMSD = 0.218429 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 7.69418 px Iteration 2: RMSD = 2.16635 px Iteration 3: RMSD = 1.05759 px Iteration 4: RMSD = 0.536397 px Iteration 5: RMSD = 0.346165 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 7.34136 px Iteration 2: RMSD = 3.65242 px Iteration 3: RMSD = 3.1586 px Iteration 4: RMSD = 3.09984 px Iteration 5: RMSD = 1.34024 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 4.4635 px Iteration 2: RMSD = 1.09902 px Iteration 3: RMSD = 0.371714 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 8.60912 px Iteration 2: RMSD = 5.56326 px Iteration 3: RMSD = 5.59495 px Iteration 4: RMSD = 5.77221 px Iteration 5: RMSD = 5.81584 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 5.66957 px Iteration 2: RMSD = 2.69635 px Iteration 3: RMSD = 2.39763 px Iteration 4: RMSD = 1.94805 px Iteration 5: RMSD = 1.423 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 4.33649 px Iteration 2: RMSD = 1.72517 px Iteration 3: RMSD = 1.38197 px Iteration 4: RMSD = 1.29888 px Iteration 5: RMSD = 1.2492 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 10.4652 px Iteration 2: RMSD = 1.5112 px Iteration 3: RMSD = 0.591838 px Iteration 4: RMSD = 0.420692 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 12.3374 px Iteration 2: RMSD = 9.13014 px Iteration 3: RMSD = 8.18749 px Iteration 4: RMSD = 7.78114 px Iteration 5: RMSD = 7.89439 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 7.54406 px Iteration 2: RMSD = 5.40608 px Iteration 3: RMSD = 1.38934 px Iteration 4: RMSD = 0.512473 px Iteration 5: RMSD = 0.36275 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 5.35749 px Iteration 2: RMSD = 1.49086 px Iteration 3: RMSD = 0.673953 px Iteration 4: RMSD = 0.301911 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 7.5393 px Iteration 2: RMSD = 1.74491 px Iteration 3: RMSD = 1.60406 px Iteration 4: RMSD = 1.51137 px Iteration 5: RMSD = 1.4814 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 3.58089 px Iteration 2: RMSD = 1.60045 px Iteration 3: RMSD = 0.706876 px Iteration 4: RMSD = 0.347801 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 7.65337 px Iteration 2: RMSD = 5.4903 px Iteration 3: RMSD = 5.42628 px Iteration 4: RMSD = 5.57912 px Iteration 5: RMSD = 5.32256 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 5.16518 px Iteration 2: RMSD = 1.75287 px Iteration 3: RMSD = 1.42975 px Iteration 4: RMSD = 0.938572 px Iteration 5: RMSD = 4.33636 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 6.64645 px Iteration 2: RMSD = 1.50101 px Iteration 3: RMSD = 1.01819 px Iteration 4: RMSD = 0.760724 px Iteration 5: RMSD = 0.229189 px Polynomial fit RMSD: X = 3.87401 px Y = 3.70884 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/139-37_001.mrc