Working on 139-152_003.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 26.8761 Std = 5.8461 Hotpixel threshold = 61.9526 Detected 252 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 2.83739 px Iteration 2: RMSD = 0.247256 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 3.08178 px Iteration 2: RMSD = 0.63442 px Iteration 3: RMSD = 0.202464 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 3.81987 px Iteration 2: RMSD = 0.853879 px Iteration 3: RMSD = 0.392043 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 4.43709 px Iteration 2: RMSD = 4.48276 px Iteration 3: RMSD = 0.903591 px Iteration 4: RMSD = 0.342164 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 12.0081 px Iteration 2: RMSD = 8.96151 px Iteration 3: RMSD = 5.77159 px Iteration 4: RMSD = 1.47665 px Iteration 5: RMSD = 0.933986 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 10.0558 px Iteration 2: RMSD = 8.37735 px Iteration 3: RMSD = 10.1305 px Iteration 4: RMSD = 8.88473 px Iteration 5: RMSD = 9.09359 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 3.28612 px Iteration 2: RMSD = 0.776576 px Iteration 3: RMSD = 0.283372 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 3.12422 px Iteration 2: RMSD = 1.11344 px Iteration 3: RMSD = 0.605022 px Iteration 4: RMSD = 0.252889 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 3.80841 px Iteration 2: RMSD = 0.809591 px Iteration 3: RMSD = 0.392695 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 12.3419 px Iteration 2: RMSD = 10.3486 px Iteration 3: RMSD = 11.9444 px Iteration 4: RMSD = 10.5197 px Iteration 5: RMSD = 8.81778 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 38.4513 px Iteration 2: RMSD = 12.129 px Iteration 3: RMSD = 8.68532 px Iteration 4: RMSD = 8.42845 px Iteration 5: RMSD = 7.27494 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 2.18125 px Iteration 2: RMSD = 0.512824 px Iteration 3: RMSD = 0.114094 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 2.51313 px Iteration 2: RMSD = 0.899441 px Iteration 3: RMSD = 0.273942 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 3.74642 px Iteration 2: RMSD = 2.41282 px Iteration 3: RMSD = 2.24321 px Iteration 4: RMSD = 0.420083 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 8.02638 px Iteration 2: RMSD = 3.04493 px Iteration 3: RMSD = 0.776088 px Iteration 4: RMSD = 0.365861 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 11.2165 px Iteration 2: RMSD = 9.54258 px Iteration 3: RMSD = 8.04492 px Iteration 4: RMSD = 8.63886 px Iteration 5: RMSD = 9.78776 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 2.4233 px Iteration 2: RMSD = 0.656671 px Iteration 3: RMSD = 0.199206 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 2.58406 px Iteration 2: RMSD = 0.517198 px Iteration 3: RMSD = 0.230086 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 3.73899 px Iteration 2: RMSD = 0.797432 px Iteration 3: RMSD = 0.394625 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 7.31134 px Iteration 2: RMSD = 1.87004 px Iteration 3: RMSD = 1.31977 px Iteration 4: RMSD = 3.73988 px Iteration 5: RMSD = 3.94547 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 5.75784 px Iteration 2: RMSD = 6.07839 px Iteration 3: RMSD = 5.95612 px Iteration 4: RMSD = 4.73024 px Iteration 5: RMSD = 4.67456 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 2.46515 px Iteration 2: RMSD = 0.355322 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 4.21315 px Iteration 2: RMSD = 0.565056 px Iteration 3: RMSD = 0.330666 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 2.42201 px Iteration 2: RMSD = 0.505165 px Iteration 3: RMSD = 0.166678 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 4.43716 px Iteration 2: RMSD = 1.25392 px Iteration 3: RMSD = 1.00443 px Iteration 4: RMSD = 0.732833 px Iteration 5: RMSD = 0.798042 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 7.67148 px Iteration 2: RMSD = 5.11753 px Iteration 3: RMSD = 5.41663 px Iteration 4: RMSD = 5.24122 px Iteration 5: RMSD = 4.38319 px Polynomial fit RMSD: X = 2.66059 px Y = 3.14218 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/139-152_003.mrc