Working on 139-144_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 28.8835 Std = 5.76769 Hotpixel threshold = 63.4896 Detected 287 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 5.0729 px Iteration 2: RMSD = 0.61688 px Iteration 3: RMSD = 0.195319 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 13.8697 px Iteration 2: RMSD = 11.978 px Iteration 3: RMSD = 12.7789 px Iteration 4: RMSD = 11.0755 px Iteration 5: RMSD = 10.9554 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 56.1992 px Iteration 2: RMSD = 11.0414 px Iteration 3: RMSD = 8.21301 px Iteration 4: RMSD = 7.50418 px Iteration 5: RMSD = 7.37287 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 45.6818 px Iteration 2: RMSD = 10.7564 px Iteration 3: RMSD = 9.70683 px Iteration 4: RMSD = 10.355 px Iteration 5: RMSD = 10.4251 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 3.60382 px Iteration 2: RMSD = 3.20087 px Iteration 3: RMSD = 3.68768 px Iteration 4: RMSD = 3.27368 px Iteration 5: RMSD = 1.3445 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 2.81562 px Iteration 2: RMSD = 1.21313 px Iteration 3: RMSD = 0.390461 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 45.1986 px Iteration 2: RMSD = 42.5823 px Iteration 3: RMSD = 41.7433 px Iteration 4: RMSD = 43.9329 px Iteration 5: RMSD = 43.702 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 17.7837 px Iteration 2: RMSD = 16.2972 px Iteration 3: RMSD = 18.8395 px Iteration 4: RMSD = 16.5251 px Iteration 5: RMSD = 7.77683 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 10.4058 px Iteration 2: RMSD = 9.48628 px Iteration 3: RMSD = 7.83561 px Iteration 4: RMSD = 5.57191 px Iteration 5: RMSD = 5.51222 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 9.14357 px Iteration 2: RMSD = 6.86349 px Iteration 3: RMSD = 1.17167 px Iteration 4: RMSD = 0.559004 px Iteration 5: RMSD = 0.274972 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 4.13362 px Iteration 2: RMSD = 1.47781 px Iteration 3: RMSD = 1.36929 px Iteration 4: RMSD = 1.33848 px Iteration 5: RMSD = 1.31325 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 20.2092 px Iteration 2: RMSD = 20.4623 px Iteration 3: RMSD = 19.3972 px Iteration 4: RMSD = 17.9405 px Iteration 5: RMSD = 18.8947 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 14.3361 px Iteration 2: RMSD = 13.8394 px Iteration 3: RMSD = 12.9389 px Iteration 4: RMSD = 13.7072 px Iteration 5: RMSD = 13.8948 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 12.4833 px Iteration 2: RMSD = 7.96836 px Iteration 3: RMSD = 3.88384 px Iteration 4: RMSD = 3.54923 px Iteration 5: RMSD = 2.84976 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 6.43899 px Iteration 2: RMSD = 8.27355 px Iteration 3: RMSD = 8.06606 px Iteration 4: RMSD = 8.16675 px Iteration 5: RMSD = 8.19163 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 3.60509 px Iteration 2: RMSD = 1.21029 px Iteration 3: RMSD = 0.469813 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 16.1383 px Iteration 2: RMSD = 12.9237 px Iteration 3: RMSD = 14.3087 px Iteration 4: RMSD = 14.1979 px Iteration 5: RMSD = 14.1923 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 14.0385 px Iteration 2: RMSD = 7.54268 px Iteration 3: RMSD = 8.91892 px Iteration 4: RMSD = 10.1492 px Iteration 5: RMSD = 11.4753 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 10.3715 px Iteration 2: RMSD = 5.21185 px Iteration 3: RMSD = 3.07588 px Iteration 4: RMSD = 3.03867 px Iteration 5: RMSD = 3.00017 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 3.44204 px Iteration 2: RMSD = 0.864001 px Iteration 3: RMSD = 0.392872 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 2.01878 px Iteration 2: RMSD = 0.429302 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 13.363 px Iteration 2: RMSD = 8.86923 px Iteration 3: RMSD = 5.39092 px Iteration 4: RMSD = 3.9978 px Iteration 5: RMSD = 0.719525 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 8.90365 px Iteration 2: RMSD = 5.64499 px Iteration 3: RMSD = 5.58746 px Iteration 4: RMSD = 5.82171 px Iteration 5: RMSD = 3.10646 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 3.63935 px Iteration 2: RMSD = 1.48837 px Iteration 3: RMSD = 0.895888 px Iteration 4: RMSD = 0.468843 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 2.89077 px Iteration 2: RMSD = 0.99605 px Iteration 3: RMSD = 0.223243 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 4.29219 px Iteration 2: RMSD = 0.719956 px Iteration 3: RMSD = 0.234828 px Polynomial fit RMSD: X = 1.98969 px Y = 2.98007 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/139-144_002.mrc