Working on 139-108_002.tif with 10 thread(s). Movie size: X = 3838 Y = 3710 N = 80 Frames to be used: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Frame grouping: n_frames = 80, requested group size = 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | interpolate_shifts = 0 In unaligned sum, Mean = 29.0172 Std = 5.73075 Hotpixel threshold = 63.4016 Detected 278 hot pixels to be corrected. Fixed hot pixels. Global alignment: Iteration 1: RMSD = 8.09071 px Iteration 2: RMSD = 0.896736 px Iteration 3: RMSD = 0.349061 px Local alignments: Patches: X = 5 Y = 5 Patch (1, 1): 1 / 25, X range = [0, 766), Y range = [0, 742), Center = (382, 370) Iteration 1: RMSD = 14.2152 px Iteration 2: RMSD = 8.98042 px Iteration 3: RMSD = 11.1022 px Iteration 4: RMSD = 2.0311 px Iteration 5: RMSD = 0.715204 px Patch (1, 2): 2 / 25, X range = [767, 1533), Y range = [0, 742), Center = (1149, 370) Iteration 1: RMSD = 46.8193 px Iteration 2: RMSD = 12.825 px Iteration 3: RMSD = 10.6461 px Iteration 4: RMSD = 9.78461 px Iteration 5: RMSD = 9.81168 px Patch (1, 3): 3 / 25, X range = [1534, 2300), Y range = [0, 742), Center = (1916, 370) Iteration 1: RMSD = 9.49657 px Iteration 2: RMSD = 6.56995 px Iteration 3: RMSD = 4.38208 px Iteration 4: RMSD = 3.93691 px Iteration 5: RMSD = 3.85512 px Patch (1, 4): 4 / 25, X range = [2301, 3067), Y range = [0, 742), Center = (2683, 370) Iteration 1: RMSD = 7.84365 px Iteration 2: RMSD = 5.0245 px Iteration 3: RMSD = 0.787758 px Iteration 4: RMSD = 0.268864 px Patch (1, 5): 5 / 25, X range = [3068, 3834), Y range = [0, 742), Center = (3450, 370) Iteration 1: RMSD = 5.38693 px Iteration 2: RMSD = 4.70196 px Iteration 3: RMSD = 6.49706 px Iteration 4: RMSD = 4.61539 px Iteration 5: RMSD = 4.63239 px Patch (2, 1): 6 / 25, X range = [0, 766), Y range = [742, 1484), Center = (382, 1112) Iteration 1: RMSD = 17.1826 px Iteration 2: RMSD = 16.278 px Iteration 3: RMSD = 16.1225 px Iteration 4: RMSD = 13.1142 px Iteration 5: RMSD = 10.6853 px Patch (2, 2): 7 / 25, X range = [767, 1533), Y range = [742, 1484), Center = (1149, 1112) Iteration 1: RMSD = 8.10664 px Iteration 2: RMSD = 4.47354 px Iteration 3: RMSD = 1.4326 px Iteration 4: RMSD = 3.31283 px Iteration 5: RMSD = 0.561607 px Patch (2, 3): 8 / 25, X range = [1534, 2300), Y range = [742, 1484), Center = (1916, 1112) Iteration 1: RMSD = 19.3279 px Iteration 2: RMSD = 6.95608 px Iteration 3: RMSD = 6.36704 px Iteration 4: RMSD = 6.39929 px Iteration 5: RMSD = 6.31712 px Patch (2, 4): 9 / 25, X range = [2301, 3067), Y range = [742, 1484), Center = (2683, 1112) Iteration 1: RMSD = 7.31349 px Iteration 2: RMSD = 2.03197 px Iteration 3: RMSD = 0.601286 px Iteration 4: RMSD = 0.285158 px Patch (2, 5): 10 / 25, X range = [3068, 3834), Y range = [742, 1484), Center = (3450, 1112) Iteration 1: RMSD = 4.27146 px Iteration 2: RMSD = 1.05469 px Iteration 3: RMSD = 0.395117 px Patch (3, 1): 11 / 25, X range = [0, 766), Y range = [1484, 2226), Center = (382, 1854) Iteration 1: RMSD = 17.3958 px Iteration 2: RMSD = 18.3045 px Iteration 3: RMSD = 13.854 px Iteration 4: RMSD = 11.7757 px Iteration 5: RMSD = 11.3747 px Patch (3, 2): 12 / 25, X range = [767, 1533), Y range = [1484, 2226), Center = (1149, 1854) Iteration 1: RMSD = 7.66457 px Iteration 2: RMSD = 10.4523 px Iteration 3: RMSD = 6.4268 px Iteration 4: RMSD = 4.54431 px Iteration 5: RMSD = 6.6899 px Patch (3, 3): 13 / 25, X range = [1534, 2300), Y range = [1484, 2226), Center = (1916, 1854) Iteration 1: RMSD = 12.722 px Iteration 2: RMSD = 5.72357 px Iteration 3: RMSD = 7.83826 px Iteration 4: RMSD = 7.67859 px Iteration 5: RMSD = 7.65986 px Patch (3, 4): 14 / 25, X range = [2301, 3067), Y range = [1484, 2226), Center = (2683, 1854) Iteration 1: RMSD = 11.9093 px Iteration 2: RMSD = 2.25809 px Iteration 3: RMSD = 1.3655 px Iteration 4: RMSD = 0.931148 px Iteration 5: RMSD = 0.878068 px Patch (3, 5): 15 / 25, X range = [3068, 3834), Y range = [1484, 2226), Center = (3450, 1854) Iteration 1: RMSD = 4.20351 px Iteration 2: RMSD = 1.08727 px Iteration 3: RMSD = 0.320062 px Patch (4, 1): 16 / 25, X range = [0, 766), Y range = [2226, 2968), Center = (382, 2596) Iteration 1: RMSD = 10.8464 px Iteration 2: RMSD = 4.10456 px Iteration 3: RMSD = 5.49907 px Iteration 4: RMSD = 0.95614 px Iteration 5: RMSD = 0.73031 px Patch (4, 2): 17 / 25, X range = [767, 1533), Y range = [2226, 2968), Center = (1149, 2596) Iteration 1: RMSD = 8.6862 px Iteration 2: RMSD = 7.12955 px Iteration 3: RMSD = 4.62046 px Iteration 4: RMSD = 4.67826 px Iteration 5: RMSD = 4.65437 px Patch (4, 3): 18 / 25, X range = [1534, 2300), Y range = [2226, 2968), Center = (1916, 2596) Iteration 1: RMSD = 10.4477 px Iteration 2: RMSD = 8.49963 px Iteration 3: RMSD = 2.69564 px Iteration 4: RMSD = 2.62237 px Iteration 5: RMSD = 2.30525 px Patch (4, 4): 19 / 25, X range = [2301, 3067), Y range = [2226, 2968), Center = (2683, 2596) Iteration 1: RMSD = 8.7615 px Iteration 2: RMSD = 2.31384 px Iteration 3: RMSD = 0.529739 px Iteration 4: RMSD = 0.236604 px Patch (4, 5): 20 / 25, X range = [3068, 3834), Y range = [2226, 2968), Center = (3450, 2596) Iteration 1: RMSD = 6.1136 px Iteration 2: RMSD = 0.968682 px Iteration 3: RMSD = 0.316319 px Patch (5, 1): 21 / 25, X range = [0, 766), Y range = [2968, 3710), Center = (382, 3338) Iteration 1: RMSD = 9.67218 px Iteration 2: RMSD = 9.34575 px Iteration 3: RMSD = 8.33639 px Iteration 4: RMSD = 8.36063 px Iteration 5: RMSD = 8.54749 px Patch (5, 2): 22 / 25, X range = [767, 1533), Y range = [2968, 3710), Center = (1149, 3338) Iteration 1: RMSD = 6.96124 px Iteration 2: RMSD = 5.24468 px Iteration 3: RMSD = 5.17909 px Iteration 4: RMSD = 5.13908 px Iteration 5: RMSD = 5.10581 px Patch (5, 3): 23 / 25, X range = [1534, 2300), Y range = [2968, 3710), Center = (1916, 3338) Iteration 1: RMSD = 7.79386 px Iteration 2: RMSD = 1.95278 px Iteration 3: RMSD = 1.14474 px Iteration 4: RMSD = 0.886093 px Iteration 5: RMSD = 0.748452 px Patch (5, 4): 24 / 25, X range = [2301, 3067), Y range = [2968, 3710), Center = (2683, 3338) Iteration 1: RMSD = 7.28079 px Iteration 2: RMSD = 2.86218 px Iteration 3: RMSD = 1.84755 px Iteration 4: RMSD = 1.57218 px Iteration 5: RMSD = 1.55536 px Patch (5, 5): 25 / 25, X range = [3068, 3834), Y range = [2968, 3710), Center = (3450, 3338) Iteration 1: RMSD = 2.50454 px Iteration 2: RMSD = 0.369478 px Polynomial fit RMSD: X = 2.70426 px Y = 3.2482 px Summing frames after dose weighting: ................................................................................ done Written aligned and dose-weighted sum to MotionCorr/job002/139-108_002.mrc