REL 2023-08-22 2023-09-05 2023-09-05 Benchmark SBF SEM data of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT Domart MC Collinson LM The Francis Crick Institute FC001999 United Kingdom 39.2 10.6019/EMPIAR-11666 CLEM cell Krentzel D Elphick M Domart M Peddie CJ Laine RF Henriques R Collinson LM Jones ML CLEM-Reg: An automated point cloud based registration algorithm for correlative light and volume electron microscopy bioRxiv 2023 English 10.1101/2023.05.11.540445
Raw and processed benchmark SBF SEM data of HeLa cells matching Airyscan light microscopy data deposited on Biostudies
 S-BSST1175 EMPIAR-10819 EMPIAR-11537 Low magnification raw SBF SEM overview images (ROI00) of the region around the cell imaged in ROI01 /data/EMPIAR/Raw/ROI_00 micrographs - single frame DM4 DM4 138 1 UNSIGNED 16 BIT INTEGER 2000 752.916 2000 752.916
SBF SEM images from a Gatan 3View on a Zeiss Sigma SEM. Low magnification consecutive overview images (ROI00) linked to higher magnification images in ROI01. 50nm thick slices. Imaged at 2kV with a 20um aperture, 2us dwell, 65% then 70% FCC after slice 26.
 Low magnification raw SBF SEM overview images (ROI00) of HeLa cell previously imaged by Airyscan /data/EMPIAR/Raw/ROI_01 micrographs - single frame DM4 DM4 138 1 UNSIGNED 16 BIT INTEGER 9000 70.148 9000 70.148
SBF SEM images from a Gatan 3View on a Zeiss Sigma SEM. High magnification images (ROI01) linked to lower magnification images in ROI00. 50nm thick slices. Imaged at 2kV with a 20um aperture, 2us dwell, 65% then 70% FCC after slice 26.
 High magnification aligned full resolution SBF SEM image stack /data/EMPIAR/Aligned-7nm micrographs - multiframe TIFF TIFF 1 133 UNSIGNED BYTE 10717 70.148 9845 70.148
Processed and aligned (in TrakEM2) tif stack of SBF SEM images of HEla cell at full resolution (7nm XY, 50nm Z) with similar orientation to the Airyscan data available on Biostudies
 High magnification aligned downscaled to 14nm XY SBF SEM image stack /data/EMPIAR/Aligned-14nm micrographs - multiframe TIFF TIFF 1 133 UNSIGNED BYTE 5359 140.296 4923 140.296
Processed and aligned tif stack of SBF SEM images downscaled (14nm XY, 50nm Z) with similar orientation to the Airyscan data available on Biostudies
 High magnification aligned downscaled to 28nm XY SBF SEM image stack /data/EMPIAR/Aligned-28nm micrographs - multiframe TIFF TIFF 1 133 UNSIGNED BYTE 2679 280.593 2461 280.593
Processed and aligned tif stack of SBF SEM images downscaled 28nm XY, 50nm Z) with similar orientation to the Airyscan data available on Biostudies