REL 2023-06-28 2024-11-08 2024-11-08 Automethylated PRC2 dimer bound to nucleosome 0000-0001-7204-5863 Paul Victor Sauer QB3 Institute, University of California, Berkeley, CA, USA Berkeley CA United States 94720 0000-0002-8304-4062 Simon Poepsel Center for Molecular Medicine Cologne (CMMC) University of Cologne Robert-Koch-Str. 21 Cologne Germany 50931 0000-0001-9816-3681 Eva Nogales Molecular and Cell Biology Department, UC Berkeley Berkeley CA United States 94720 Sauer PV Pavlenko E Nogales E Poepsel S Cookis T 1.8 10.6019/EMPIAR-11607 EMDB molecule EMD-41110 EMD-41141 EMD-41146 Sauer PV Pavlenko E Cookis T Zirden LC Renn J Singhal A Hunold P Hoehne-Wiechmann MN van Ray O Kaschani F Kaiser M Hänsel-Hertsch R Sanbonmatsu KY Nogales E Poepsel S Activation of automethylated PRC2 by dimerization on chromatin Molecular cell Mol Cell 2024 English 10.1016/j.molcel.2024.08.025 39303719
Micrographs of datasets 1,2 and 4 from the paper. PRC2 dimer bound to nucleosome, PRC2 monomer bound to nucleosome, PRC2-J119-450 monomer bound to H1-nucleosome
 Dataset1, automethylated PRC2 dimer bound to nucleosome, motion corrected, dose weighted, streptavidin subtracted /data/Dataset1_PRC2dimer micrographs - single frame MRC MRC 3894 1 32 BIT FLOAT 3710 1.15 3838 1.15
Motion corrected, dose weighted, streptavidin subtracted, dose 40 electron per square angstrom
 Dataset2, automethylated PRC2 dimer bound to nucleosome, motion corrected, dose weighted, streptavidin subtracted /data/Dataset2_PRC2dimer micrographs - single frame MRC MRC 4062 1 32 BIT FLOAT 3710 1.15 3838 1.15
Motion corrected, dose weighted, streptavidin subtracted, dose 40 electron per square angstrom
 Dataset4, PRC2_J119-450 bound to H1-nucleosome, motion corrected, dose weighted, streptavidin subtracted /data/Dataset4_PRC2-J119-450_H1nuc micrographs - single frame MRC MRC 14470 1 32 BIT FLOAT 4092 0.94 5760 0.94
Motion corrected, dose weighted, streptavidin subtracted, dose 50 electron per square angstrom
 Dataset3, H1 bound to nucleosome /data/Dataset3_H1-Nuc micrographs - single frame MRC MRC 2236 1 32 BIT FLOAT 4092 1.14 5760 1.14
The sample used here was PRC2 (EZH2, SUZ12,EED, RBAP48, AEBP2, but without Jarid2) together with H1.0 bound to nucleosome. However it didn't form a complex. Instead, this dataset yielded a structure of H1.0 bound to nucleosome alone. In addition, the quality of the streptavidin lattice was not good, so the subtraction was incomplete. Therefore, when processing this dataset there might be leftovers of streptavidin appearing in the 2D classes. This dataset was recorded on a Talos Arctica at 200kV with a dose of 50 electrons per square Angstrom.