REL
2023-06-01
2024-03-18
2024-03-18
Unseeded Aβ(1-40) amyloid fibrils
0000-0002-9442-460X
Matthias
Schmidt
Institut für Proteinbiochemie, Universität Ulm
8/1 Helmholtzstrasse
Ulm
Baden Württemberg
Germany
89081
0000-0003-2123-6816
Marcus
Fändrich
Institut für Proteinbiochemie, Universität Ulm
8/1 Helmholtzstrasse
Ulm
Baden Württemberg
Germany
89081
Pfeiffer PB
Schmidt M
Fändrich M
German Research Foundation (DFG)
FA 456/24-1
Germany
1.9
10.6019/EMPIAR-11595
EMDB
molecule
EMD-17166
EMD-17167
Pfeiffer PB
Ugrina M
Schwierz N
Sigurdson CJ
Schmidt M
Fändrich M
Cryo-EM analysis of the effect of seeding with brain-derived Aβ amyloid fibrils
Germany
English
Unaligned multi-frame micrographs of unseeded Aβ(1-40) amyloid fibrils
/data/movies
micrographs - multiframe
EER
EER
4628
918
UNSIGNED 16 BIT INTEGER
4096
0.934
4096
0.934
IC-Krios1 G4 300kV C-FEG
SelectrisX 10eV slit width, Falcon4i
Cs=2.7mm, C2aperture=50um, ObjAperture=100um
EFTEM 130kx, nanoprobe spot 5, beam=630nm
Counting mode, 1pix=0.934 A
~2.9 s exposure, EER files save (918 fractions, not gain corrected)
~40 e/A^2 total dose
defocus range: -0.6 to -2.1
The gain reference (/data/movies/20220808_133943_EER_GainReference.gain) is not applied to the micrographs. The NOTES.txt file contains EM information. Thumbnails (/data/thumbs) as well as coordinates are provided. The coordinates are divided in morphology unseeded i (/data/manual pick unseeded i) and unseeded ii (/data/manual pick unseeded ii).
data/movies/*.eer
data/manual pick unseeded */*.star
data/manual pick unseeded *