REL 2022-12-13 2024-05-29 2024-06-17 0000-0001-6209-4012 Christian Tüting Kastritis Laboratory for Biomolecular Research, Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg Weinbergweg 22 Halle Saxony-Anhalt Germany 06120 0000-0002-1463-8422 Panagiotis Kastritis Kastritis Laboratory for Biomolecular Research, Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg Weinbergweg 22 Halle Saxony-Anhalt Germany 06120 Schmidt L Tueting C Kyrilis F Hamdi F Semchonok DA Kastritis PL 23.8 10.6019/EMPIAR-11581 EMDB virus EMD-17628 Schmidt L Tüting C Kyrilis FL Hamdi F Semchonok DA Hause G Meister A Ihling C Stubbs MT Sinz A Kastritis PL Communications biology Commun Biol 1 7 2024 English 10.1038/s42003-024-06204-7 38730276 EMPIAR-11069 unaligned multi-frame micrographs of a high-molecular yeast fraction /data micrographs - multiframe EER EER 51497 972 BIT 4096 0.93 4096 0.93

This dataset provides the cryo-electron microscopy (cryo-EM) analysis of high molecular weight biomolecules isolated from Saccharomyces cerevisiae, commonly known as baker's yeast. The cells were grown, lysed, and the lysate was subsequently cleared and fractionated using size exclusion chromatography. The data focuses on fraction #5, known for its rich composition of ribosomes and L-A virus capsids. The cryo-EM data presented here offers a comprehensive visualization of these essential biomolecular structures, providing an insightful perspective into their structural organization and potentially informing future studies of yeast cellular processes and viral interactions.