<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11557" schemaVersion="0.64" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2023-03-24</depositionDate>
            <releaseDate>2024-01-16</releaseDate>
            <updateDate>2024-01-16</updateDate>
        </keyDates>
        <title>Cryo Electron tomography of germinated polar tubes from Vairimorpha necatrix</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-9518-4671</authorORCID>
            <firstName>HIMANSHU</firstName>
            <lastName>Sharma</lastName>
            <organization type="academic">Umeå University</organization>
            <street>Dept of Molecular Biology, Department of Medical Biochemistry and Biophysics, Umeå University</street>
            <townOrCity>UMEA</townOrCity>
            <stateOrProvince>Vasterbotten</stateOrProvince>
            <country>Sweden</country>
            <postOrZipCode>90130</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-2971-8190</authorORCID>
            <firstName>Jonas</firstName>
            <lastName>Barandun</lastName>
            <organization type="academic">Dept of Molecular Biology, Umeå University</organization>
            <street>Biomedicinhuset, byggnad 6 K och 6 L</street>
            <townOrCity>Umeå</townOrCity>
            <stateOrProvince>Vasterbotten</stateOrProvince>
            <country>Sweden</country>
            <postOrZipCode>90730</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-9518-4671">Sharma H</author>
            <author>Jespersen N</author>
            <author authorORCID="0000-0003-1312-5825">Ehrenbolger K</author>
            <author authorORCID="0000-0003-2342-6488">Carlson L.A.</author>
            <author authorORCID="0000-0003-2971-8190">Barandun J</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Swedish Energy Agency</fundingBody>
                <code>2019-02011</code>
                <country>Sweden</country>
            </grantReference>
            <grantReference>
                <fundingBody>European Research Council (ERC)</fundingBody>
                <code>948655</code>
                <country>Sweden</country>
            </grantReference>
            <grantReference>
                <fundingBody>European Commission</fundingBody>
                <code>MsInfection-101033469</code>
                <country>Sweden</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">94.1</datasetSize>
        <entryDOI>10.6019/EMPIAR-11557</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>cell</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-17391</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <nonJournalCitation published="false">
                    <author authorORCID="0000-0001-9518-4671" order="1">Sharma H</author>
                    <author order="2">Jespersen N</author>
                    <author authorORCID="0000-0003-1312-5825" order="3">Ehrenbolger K</author>
                    <author authorORCID="0000-0003-2342-6488" order="4">Carlson L A</author>
                    <author authorORCID="0000-0003-2971-8190" order="5">Barandun J</author>
                    <bookTitle>Cryo Electron tomography of germinated polar tubes from Vairimorpha necatrix</bookTitle>
                    <volume>na</volume>
                    <publisher>bioRxiv</publisher>
                    <publicationLocation>Preprint</publicationLocation>
                    <country></country>
                    <firstPage>na</firstPage>
                    <lastPage>na</lastPage>
                    <year>2023</year>
                    <language>English</language>
                    <details>Raw tilt series collected on Titan krios using a Gatan K2 Bioquantum direct electron detector. Collection was done at tilt angle range of -60 to +60 degrees with a total exposure for the tilt series is 110-120 e/Ang2.</details>
                </nonJournalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Motion corrected, unaligned tilt series stack.</name>
        <directory>/data/tiltseries</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>32</numImagesOrTiltSeries>
        <framesPerImage>8</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>2.173</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>2.173</pixelHeight>
        </dimensions>
        <details>Motion-corrected raw tilt series and representative tomograms for cargo-filled and empty polar tubes are uploaded.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Representative tomograms of cargo filled and empty tubes.</name>
        <directory>/data</directory>
        <category>reconstructed volumes</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>2</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>959</imageWidth>
            <pixelWidth>8.69</pixelWidth>
            <imageHeight>256</imageHeight>
            <pixelHeight>8.69</pixelHeight>
        </dimensions>
        <details>Reconstructed representative tomograms showing germinated polar tubes.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
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