REL 2023-04-27 2024-04-30 2024-04-30 0000-0002-7397-1321 Martin Beck Max Planck Institute of Biophysics Max-von-Laue-Str. 3 Frankfurt Hessen Germany 60438 0000-0002-7397-1321 Martin Beck Max Planck Institute of Biophysics Max-von-Laue-Str. 3 Frankfurt Hessen Germany 60438 Xing H Welsch S Turonova B Beck M Max Planck Society none Germany 1.3 10.6019/EMPIAR-11538 EMDB cell EMD-16721 Xing H Welsch S Turoňová B Beck M Tilt-series of FIB-milled HEK293T cells under native and HHT-treated conditions /data tilt series MRC MRC 710 59 SIGNED 16 BIT INTEGER 4096 1.188 4096 1.188

All mdoc files corresponding to the tilt-series are provided. Tilt series were acquired on a Titan Krios G4 (Thermo Fisher Scientific) operated at 300kV and equipped with Selectris X imaging filter and Falcon 4 direct electron detector, at 4K x 4K pixel dimensions, tilt range of -60° to 60°, 2° tilt increment, target defocus of -1.5 to -4.5 μm and a total dose of 120 to 150 e/Å2 per tilt series, using SerialEM software. There are 30-61 tilts per tilt series, which depends on when the tilt series data collection finished or failed. Target areas were selected in the cytoplasmic region of the cell.