Benchmark FIB SEM data (#2) of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy

REL 2023-04-27 2023-05-22 2023-05-23 Benchmark FIB SEM data (#2) of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT Peddie CJ Domart MC Collinson LM The Francis Crick Institute FC001999 United Kingdom 511.9 10.6019/EMPIAR-11537 CLEM cell Krentzel D Elphick M Domart M Peddie CJ Laine RF Henriques R Collinson LM Jones ML CLEM-Reg: An automated point cloud based registration algorithm for correlative light and volume electron microscopy bioRxiv 2023 English 10.1101/2023.05.11.540445

Raw and processed benchmark FIB SEM data matching Airyscan data deposited on Biostudies

S-BSST1075 EMPIAR-10819 Raw FIB SEM Run 1 /data/4G_Run 1 micrographs - single frame TIFF TIFF 3419 1 UNSIGNED 16 BIT INTEGER 14976 50.0 14976 50.0

First part of the raw data (out of 3 in total). Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Run 2 /data/4G_Run 2 micrographs - single frame TIFF TIFF 156 1 UNSIGNED 16 BIT INTEGER 14976 50.0 14976 50.0

Second part of the raw data (out of 3 in total). Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Run 3 /data/Run 3 micrographs - single frame TIFF TIFF 4266 1 UNSIGNED 16 BIT INTEGER 14976 50.0 14976 50.0

Third part of the raw data (out of 3 in total). Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Downscaled (20nm) aligned FIB SEM stack resliced to match the Airyscan dataset /data/Downscaled20nm micrographs - multiframe TIFF TIFF 1 258 UNSIGNED BYTE 3213 200.0 1959 200.0

Processed and aligned stack of FIB SEM images downscaled to 20 nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies

Aligned full resolution FIB SEM stack resliced to match the Airyscan dataset /data/5nm micrographs - multiframe TIFF TIFF 1 900 UNSIGNED BYTE 12850 50.0 7835 50.0

Processed and aligned stack of FIB SEM images at full 5nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies