REL
2023-03-31
2024-03-18
2024-03-18
Ex vivo murine AApoAII amyloid fibril
0000-0002-9442-460X
Matthias
Schmidt
Institut für Proteinbiochemie, Universität Ulm
8/1 Helmholtzstrasse
Ulm
Baden Württemberg
Germany
89081
0000-0003-2123-6816
Marcus
Fändrich
Institut für Proteinbiochemie, Universität Ulm
8/1 Helmholtzstrasse
Ulm
Baden Württemberg
Germany
89081
Andreotti S
Schmidt M
Fändrich M
German Research Foundation (DFG)
FA 456/27-1
Germany
German Research Foundation (DFG)
FA 456/28-1
Germany
510.6
10.6019/EMPIAR-11525
EMDB
molecule
EMD-17105
EMD-17104
Andreotti G
Baur J
Ugrina M
Pfeiffer PB
Hartmann M
Wiese S
Miyahara H
Higuchi K
Schwierz N
Schmidt M
Fändrich M
Insights into the Structural Basis of Amyloid Resistance Provided by Cryo-EM Structures of AApoAII Amyloid Fibrils
Journal of molecular biology
J Mol Biol
Germany
4
436
2024
English
10.1016/j.jmb.2024.168441
38199491
Unaligned multi-frame micrographs of ex vivo murine AApoAII fibrils
/data/data
micrographs - multiframe
TIFF
TIFF
3083
40
UNSIGNED 16 BIT INTEGER
3838
variable
3710
variable
Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 620nm
c2=50 objective_aperture=100
GIF-K2 counting
12s exposure 40 frames
total dose at the sample 42.70e/A2
1 pixel = 1.04A
dose rate ~3 e/pix/s
defocus range -1.2 to -2.5
Gain reference is already applied to the micrographs and the NOTE.txt contains the EM information.
Thumbnails and power spectra (/data/thumbs) are provided, as well as the defect files (/data/data).
The ManualPick folder contains the coordinates for two different fibril morphologies. Coordinates files are divided in part_1 and part_2 for each morphologies and they are connected to micrographs with different defect files.
data/data/*.tif
data/ManualPick/Morphology_*/part_*/*_manualpick.star
data/ManualPick