REL 2023-04-18 2024-11-06 2024-11-06 0000-0003-4368-2526 Liang Xue European Molecular Biology Laboratory(EMBL) Meyerhofstrasse 1 Heidelberg Germany (DEU) Germany 69126 0000-0001-6968-041X Julia Mahamid European Molecular Biology Laboratory(EMBL) Meyerhofstrasse 1 Heidelberg Germany (DEU) Germany 69126 Xue L Spahn CMT Schacherl M Mahamid J Chan Zuckerberg Initiative Visual Proteomics Germany 170.5 10.6019/EMPIAR-11520 EMDB cell EMD-17132 Xue L Spahn CMT Schacherl M Mahamid J Mahamid J Germany 2023 English

Dose-symmetric tilt series of intact M. pneumoniae cells treated with chloramphenicol. Gold fiducials were added before plunge freezing. Data were collected on a Titan Krios microscope equipped with Gatan K3 camera (Magnification 64,000x, pixel size 1.329 Å, tilt range -60°:3°:60°, camera in non-CDS counting mode, 10 frames per tilt image, constant exposure time for each tilt and total dose 137 e/Å2, defocus range from -1 to -3.25μm). Raw frames (movie) for every tilt images of the 139 tilt series were deposited. Gain reference needs flipping along the Y axis in Warp. Detailed information can be found in the provided mdoc files. IMOD/etomo alignment files are also provided (preferentially with motion correction and raw data processing performed in Warp).

Unaligned multi-frame micrographs of tilt series of chloramphenicol treated Mycoplasma pneumoniae cells /data/Cm_K3_data/frames micrographs - multiframe TIFF TIFF 139 10 UNSIGNED BYTE 5760 1.329 4092 1.329

5808 unaligned multiframe micrographs in LZW compressed TIFF format of 139 tilt series of chloramphenicol treated M. pneumoniae cells. Data collected with a Gatan K3 camera in non-CDS mode, magnification 64k, and 3.34 e-/pixel dose per tilt. Gain reference needs flipping along the Y axis. SerialEM mdoc files and the IMOD/etomo alignment files (preferentially with motion correction and raw data processing performed in Warp) are provided.