REL
2023-03-28
2024-08-26
2024-08-27
Cryo-EM structure of human pseudouridine synthase 3
0000-0003-2815-7133
Sebastian
Glatt
Malopolska Centrum Biotechnology
Krakow
malopolska
Poland
30-387
0000-0001-7914-2164
Ting-Yu
Lin
Jagiellonian University Krakow
gronostajowa 7a
Krakow
malopolska
Poland
30-387
Lin T.-Y.
Glatt S.
Polish National Science Centre
2019/35/D/NZ1/02397
Poland
European Research Council (ERC)
101001394
Poland
3.4
10.6019/EMPIAR-11510
EMDB
molecule
EMD-16917
Lin TY
Kleemann L
Jeżowski J
Dobosz D
Rawski M
Indyka P
Ważny G
Mehta R
Chramiec-Głąbik A
Koziej Ł
Ranff T
Fufezan C
Wawro M
Kochan J
Bereta J
Leidel SA
Glatt S
The molecular basis of tRNA selectivity by human pseudouridine synthase 3
Molecular cell
Mol Cell
13
84
2472
2489.e8
2024
English
10.1016/j.molcel.2024.06.013
38996458
Human PUS3 apo structure
/data/data
micrographs - single frame
TIFF
TIFF
8211
40
UNSIGNED BYTE
variable
variable
variable
variable
high tension 300kV
spherical aberration 2.7mm
pixel size 0.86 A/px
total dose on vacuum 40.06 e/A2
frames per movie 40
defocus range set -1.5, -1.2, -0.9 um
sample tilt 0 deg
imaging mode EFTEM
magnification of hole image 11500x
magnification of exposure image 105000x
beam size 0.75 um
C2 aperture 50um
objective aperture retracted
Gatan BioQuantum energy filter
energy slit 20eV
Gatan K3 counting mode
dose rate on vacuum 16.35 e/px/s
exposure image resolution 5760 x 4092
physical pixel (Super Resolution off)
image type .tiff (gain corrected)
EPU version 2.10.0.1941REL