REL 2022-12-06 2023-09-26 2023-09-26 0000-0003-0930-4249 Arnaud Vanden Broeck Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University 1230 York Avenue New York NY United States 10065 0000-0002-9373-4737 Sebastian Klinge Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University 1230 York Avenue New York NY United States 10065 Vanden Broeck A Klinge S European Molecular Biology Organization (EMBO) ALTF 711-2019 Germany The G. Harold and Leila Y. Mathers Foundation MF-2104-01554 United States 140.5 10.6019/EMPIAR-11472 EMDB molecule EMD-29104 EMD-29252 EMD-29105 EMD-29253 EMD-29106 EMD-29254 EMD-29107 EMD-29255 EMD-29108 EMD-29256 EMD-29109 EMD-29257 EMD-29110 EMD-29258 EMD-29111 EMD-29259 EMD-29112 EMD-29260 EMD-29113 EMD-29261 EMD-29114 EMD-29262 EMD-29115 EMD-29263 EMD-29130 EMD-29131 EMD-29132 EMD-29133 EMD-29134 EMD-29135 EMD-29136 EMD-29137 EMD-29138 EMD-29139 EMD-29140 EMD-29141 EMD-29142 EMD-29128 EMD-29129 EMD-29143 EMD-29144 EMD-29145 EMD-29146 EMD-29147 EMD-29148 EMD-29149 EMD-29150 EMD-29151 EMD-29152 EMD-29153 EMD-29154 EMD-29155 EMD-29156 EMD-29157 EMD-29158 EMD-29159 EMD-29160 EMD-29161 EMD-29162 EMD-29163 EMD-29164 EMD-29165 EMD-29166 EMD-29167 EMD-29168 EMD-29169 EMD-29170 EMD-29171 EMD-29173 EMD-29174 EMD-29175 EMD-29176 EMD-29177 EMD-29178 EMD-29179 EMD-29180 EMD-29181 EMD-29182 EMD-29183 EMD-29184 EMD-29185 EMD-29186 EMD-29187 EMD-29188 EMD-29189 EMD-29192 EMD-29194 EMD-29193 EMD-29116 EMD-29265 EMD-29117 EMD-29266 EMD-29118 EMD-29267 EMD-29119 EMD-29268 EMD-29120 EMD-29269 EMD-29121 EMD-29271 EMD-29122 EMD-29272 EMD-29123 EMD-29273 EMD-29124 EMD-29274 EMD-29125 EMD-29275 EMD-29126 EMD-29276 EMD-29127 EMD-29277 EMD-29195 EMD-29196 EMD-29197 EMD-29198 EMD-29199 EMD-29200 EMD-29201 EMD-29202 EMD-29204 EMD-29205 EMD-29206 Vanden Broeck A Klinge S Science (New York, N.Y.) Science 6653 381 2023 English 10.1126/science.adh3892 37410842 Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 1 /data/Tifs_DS1_MK67I-GFP micrographs - multiframe TIFF TIFF 44352 40 1 40 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 2 /data/Tifs_DS2_MK67I-GFP micrographs - multiframe TIFF TIFF 45846 40 1 40 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 3 /data/Tifs_DS3_MK67I-GFP micrographs - multiframe TIFF TIFF 44335 40 1 40 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 4 /data/Tifs_DS4_MK67I-GFP micrographs - multiframe TIFF TIFF 37166 40 1 40 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Polished particle stack of human nucleolar and nuclear pre-60S assembly intermediates /data/Particle_stack micrographs - single frame MRCS MRCS 2474967 1 32 BIT FLOAT 480 1.08 480 1.08

Merged set of pre-60S particles from four datasets. Particles are polished, magnification anisotropy corrected and per-particle defocus corrected. A Relion .star format file is provided and contains the final Euler angles, shifts and also the micrograph coordinates.