REL 2023-02-10 2023-07-10 2023-07-10 0000-0002-4406-5151 Camilla Ventura Santos MRC Laboratory of Molecular Biology Cambridge Biomedical Campus, Francis Crick Ave Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0001-7292-5430 Andrew Carter MRC Laboratory of Molecular Biology Cambridge Biomedical Campus, Francis Crick Ave Cambridge Cambridgeshire United Kingdom CB2 0QH Ventura Santos C Carter AP Rogers SL Medical Research Council (MRC, United Kingdom) MC_UP_A025_1011 United Kingdom Welch Foundation 210711/Z/18/Z United Kingdom 446.0 10.6019/EMPIAR-11450 EMDB cell EMD-16685 EMD-16693 Ventura Santos C Rogers SL Carter AP bioRxiv 2023 10.1101/2023.03.31.535077 Dataset 1: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells /data/Dataset1_210428/Raw_Movies micrographs - multiframe TIFF TIFF 25 10 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 1. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.

Dataset 1: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions /data/Dataset1_210428/bin4_deconvoled_tomograms reconstructed volumes MRC MRC 25 10 32 BIT FLOAT 1124 11.808 1124 11.808

Dataset 1: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.

Dataset 2: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells /data/Dataset2_210605DY2/raw_movies micrographs - multiframe TIFF TIFF 17 10 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 2. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.

Dataset 2: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions /data/Dataset2_210605DY2/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 17 10 32 BIT FLOAT 960 11.808 928 11.808

Dataset 2: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.

Dataset 3: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells /data/Dataset3_210725_DZ1/Raw_movies micrographs - multiframe TIFF TIFF 39 14 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 3. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.

Dataset 3: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions /data/Dataset3_210725_DZ1/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 39 14 32 BIT FLOAT 960 11.808 928 11.808

Dataset 3: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.

Dataset 4: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells /data/Dataset4_210724FB34/raw_movies micrographs - multiframe TIFF TIFF 22 14 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 4. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.

Dataset 4: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions /data/Dataset4_210724FB34/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 22 14 32 BIT FLOAT 960 11.808 928 11.808

Dataset 4: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.