REL 2023-01-31 2023-06-23 2023-06-23 IgG3-C1-C4b complex on a lipid bilayer 0000-0002-1990-2333 Thomas H. Sharp Department of Cell and Chemical Biology, Leiden University Medical Centre Einthovenweg 20 Leiden Zuid Holland Netherlands 2333 ZC 0000-0002-1990-2333 Thomas H. Sharp Department of Cell and Chemical Biology, Leiden University Medical Centre Einthovenweg 20 Leiden Zuid Holland Netherlands 2333 ZC Abendstein LA Sharp THS 160.1 10.6019/EMPIAR-11407 EMDB molecule EMD-16241 EMD-16251 EMD-16250 Abendstein LA Complement is activated by elevated IgG3 hexameric platforms and deposits C4b onto distinct antibody domains extracted particles - IgG3-C1-C4b complexes bound to DNP representing liposomes /data picked particles - multiframe - processed MRC MRC 2561 10 32 BIT FLOAT 256 3.48 256 3.48
Tilt series were collected on a Talos Arctica (200kV) microscope with a K3 direct electron detector and a Bioquantum energy filter (slit width=20 eV; Gatan). Tomograms were reconstructed using weighed back-projection in IMOD4.11.Particles were manually picked in EMAN2 (version 2.91) using 2spt_boxer_old.py. 2,561 particles were extracted from 101 tomograms with a box size of 256 pixels and a final pixel size of 3.48 Å/pixel.
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