REL 2023-01-13 2023-03-27 2023-03-28 0000-0003-1329-8056 Shahid Rehan Omass Therapeutics The Oxford Science Park, The Schrödinger Building, Heatley Rd Oxford Oxfordshire United Kingdom ox44sy 0000-0002-3160-7767 Ville Pavilainen HiLife Institute of Biotechnology, University of Helsinki Helsinki Finland 00790 Rehan S Academy of Finland 338836 Finland Academy of Finland 314672 Finland Academy of Finland 314669 Finland 12.9 10.6019/EMPIAR-11405 EMDB molecule EMD-14776 Rehan S Tranter D Sharp PP Lowe E Anderl JL Muchamuel T Abrishami V Kuivanen S Wenzell N Jennings A Kalyanaraman C Craven GB Strandin T Javanainen M Vapalahti O Jacobson M McMinn D Kirk CJ Huiskonen JT Taunton J Paavilainen VO bioRxiv 2022 10.1101/2022.07.03.498529 Unaligned micrographs of the ribosome/Sec61/KZR-8445 complex /data micrographs - multiframe TIFF TIFF 30260 50 1 50 UNSIGNED BYTE 11520 0.415 8184 0.415

The micrographs are the average, without any realignment of 50 movie frames (accumulating 47.66 electrons per square Ångstrom). Data was collected in super resolution mode (physical pixel size 0.83 px/Å) and binned 2x during preprocessing. Data collected on a Krios instrument with a K3 detector operated at 300 kV and at a nominal magnification of 105000x. Details: Nominal Defocus range (min, max, step): -0.6, -2.2 Total exposure on specimen: 47.66 e/A^2 Exposure time: 3.0 s Nominal pixel size: 0.83 A (0.415 A super res) Objective lens spherical aberration (Cs): 2.7 mm Acceleration voltage: 300 kV Microscope / Detector: 300 kV FEI Titan Krios TEM / Gatan K3 summit

data/KZR445_raw_mics*/FoilHole*.tiff Motion corrected micrographs of the ribosome/Sec61/KZR-8445 complex /data micrographs - single frame MRC MRC 30260 1 UNSIGNED BYTE 5760 0.83 4092 0.83

Positions of particles for the final reconstructed volume in associated .star file.

data/Motion_corrected_CTF*/KZR445_raw_mics*.mrc data/relion_model.star data/