REL 2023-01-13 2023-02-22 2023-02-22 0000-0002-2778-3936 Wanda Kukulski University of Bern Bern Switzerland 3012 0000-0002-2778-3936 Wanda Kukulski University of Bern Bern Switzerland 3012 0000-0002-7857-7032 David B Savage Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge Cambridge United Kingdom CB2 0QQ Ganeva I Lim K Boulanger J Hoffmann PC Muriel O Borgeaud AC Hagen WJH Savage DB Kukulski W 187.3 10.6019/EMPIAR-11394 EMDB cell EMD-16455 Ganeva I Lim K Boulanger J Hoffmann PC Muriel O Borgeaud AC Hagen WJH Savage DB Kukulski W Cell Reports Cell Rep. 42 2023 English 10.1016/j.celrep.2023.112107 Unaligned tilt series or dose-fractionated tilt images acquired on cryo-FIB milled HeLa cells expressing Cidec-EGFP /data/RawData tilt series MRC MRC 15 3 UNSIGNED 16 BIT INTEGER 3838 3.7 3710 3.7

Each subdirectory contains the raw data that was used for reconstruction of one electron cryo-tomogram. In 14 cases, the raw data are three unaligned, dose-fractionated frames per tilt image. In one case, the raw data is the unaligned series of tilt images. Note that pixel sizes are either 3.7 or 7.1 Angstrom. Please check header in each case. The sample are cryo-FIB milled HeLa cells expressing Cidec-EGFP.

Reconstructed electron cryo-tomograms of cryo-FIB milled HeLa cells expressing Cidec-EGFP /data/Reconstructions reconstructed volumes MRC MRC 15 3 SIGNED BYTE 1918 7.5 1854 7.5

15 reconstructed tomograms, each obtained from the tilt images contained in one subdirectory of the directory RawData. The data was binned during the tilt series alignment. The resulting pixel sizes are either 7.5 or 7.1 Angstrom. Please check header in each case. Note that image width and height vary from case to case.