REL
2022-12-12
2023-01-20
2023-01-20
Cryogenic electron tomographs of Coxsackievirus A9 treated with endosomal ionic condition buffer
0000-0001-7060-5871
Sarah
Jane
Butcher
University of Helsinki, Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences Research Programme
room 2403, Biocenter 1, Viikinkaari 9
Helsinki
Finland
00790
0000-0001-7060-5871
Sarah
Jane
Butcher
University of Helsinki, Molecular and Integrative Biosciences Research Programme, Institute of Biotechnology, Macromolecular structure and function
room 2403, Biocenter 1, Viikinkaari 9
Helsinki
Finland
00790
Domanska A
Plavec Z
Ruokolainen V
Löflund B
Marjomäki V
Butcher SJ
1.8
10.6019/EMPIAR-11364
EMDB
virus
EMD-15706
Domanska A
Plavec Z
Ruokolainen V
Löflund B
Marjomäki V
Butcher SJ
López S
Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release
Journal of virology
J Virol
24
96
2022
English
10.1128/jvi.01367-22
36448797
Micrographs of CVA9 treated with endosomal ionic condition buffer for 3 hours at 37 °C. Sample was applied to Quantifoil R1.2/1.3 holey carbon grids with 300 copper mesh and manually blotted and flash-frozen using a homemade plunger. Data was acquired at the Biocenter Finland National Cryo-EM facility with a FEI TALOS Artica transmission electron microscope operated at 200 kV. Movies were collected with a Falcon III direct electron detector at a nominal magnification of 120 000x giving a pixel size of 1.24 Å per pixel. Each movie consisted of 30 frames with an exposure time of 1 second per frame. The total electron dose was approximately 30 electrons per Å2. Movies were corrected for beam-induced motion by aligning and averaging all frames with Motioncor2 implemented in Scipion version 2.0.
Raw micrographs of CVA9 treated with ionic buffer condtions 3h
/data
micrographs - multiframe
MRC
MRC
1964
30
SIGNED 16 BIT INTEGER
4096
1.24
4096
1.24
Micrographs were collected on FEI TAlos Arctica operating at 200 kV with a Falcon III direct electron detector at a nominal magnification of ×120,000. Each movie consisted of 30 frames with an exposure time of 1 s per frame. The total electron dose was approximately 30 electrons per Å2.
data/FoilHole*.mrc
data/output_particles.star
data