REL 2022-02-21 2023-01-16 2023-01-16 0000-0001-6486-9408 Charles E Bell Department of Biological Chemistry and Pharmacology, Ohio State University 1060 Carmack Rd Columbus Oio United States 43210 0000-0001-6486-9408 Charles E Bell Department of Biological Chemistry and Pharmacology, Ohio State University 1060 Carmack Rd Columbus Oio United States 43210 Bell CE 822.3 10.6019/EMPIAR-11353 EMDB molecule EMD-26437 Caldwell BJ Norris AS Karbowski CF Wiegand AM Wysocki VH Bell CE Nature communications Nat Commun 1 13 2022 English 10.1038/s41467-022-35572-z 36543802 Unaligned multi-fram micrographs for structure of LiRecT in complex with 83-mer ssDNA /data micrographs - multiframe TIFF TIFF 1619 45 UNSIGNED BYTE 11520 0.4495 8184 0.4495

Data collected on 11/23/20-11/24/20 Krios, K3, counted mode, EFTEM, Cs corrector, super-resolution User/Group:Brian Caldwell/Chuck Bell Image information: Total # of images = 1633 selected, 22 grid squares Total data size = 0.933 TB Rate of collection = 69/hour Mode of collection = Accurate hole centering Gain normalized = NO File format = LZW tiff Super-resolution = YES Spherical aberration = 0.01 mm Images per hole = 1 Exposure time = 2.83 s Fractions = 45 Microscope parameters: Pixel size = 0.899 A Magnification = 81,000 Stage tilt = 0 deg Dose rate = 18.43 e/px/s Dose rate = 22.80 e/A^2/s Total dose = 65 e/A^2 [desired dose rate] Defocus range = -3.5, -3, -2.5, -2, -1.5, -1 C2 aperture = 50 um Objective aperture= 100 um Illuminated area = 1.5 um Energy Filter = YES Slit width = 20 eV Grid type = Quantifoil (gold coated) R1.2/1.3 300 Mesh hole size, mesh, etc. Position 1 of the large dewar Bell Conical: Position 1 of box F2 (Purple) Prepared on [date] by Brian Caldwell Additional notes: Copied gain reference file over into the data folder. Gain reference image located at: /data/gain.mrc Saved EPU settings file.

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