REL
2022-03-15
2023-01-30
2023-01-30
Structure of RecT protein from Listeria innoccua phage A118 in complex with 83-mer annealed duplex
0000-0001-6486-9408
Charles
E
Bell
Department of Biological Chemistry and Pharmacology, Ohio State University
1060 Carmack Rd
Columbus
Ohio
United States
43210
0000-0001-6486-9408
Charles
E
Bell
Department of Biological Chemistry and Pharmacology, Ohio State University
1060 Carmack Rd
Columbus
Ohio
United States
43210
Bell CE
1.6
10.6019/EMPIAR-11348
EMDB
molecule
EMD-26434
Caldwell BJ
Norris AS
Karbowski CF
Wiegand AM
Wysocki VH
Bell CE
Structure of a RecT/RedĪ² family recombinase in complex with a duplex intermediate of DNA annealing
Nature communications
Nat Commun
1
13
2022
English
10.1038/s41467-022-35572-z
36543802
Unaligned multi-frame micrographs of LiRecT in complex with 83-mer annealed duplex
/data
micrographs - multiframe
MRC
MRC
2038
36
UNSIGNED BYTE
5760
0.899
4092
0.899
Data collected on 9/17/2020-9/18-2020
Krios, K3, counted mode, EFTEM, Cs corrector
User/Group:Brian Caldwell/Chuck Bell
Image information:
Total # of images = 2038
Total data size = 1.70 TB
Rate of collection = 71/hour
Mode of collection = Accurate hole centering
Gain normalized = YES
File format = mrc
Super-resolution = NO
Spherical aberration = 0.01 mm
Images per hole = 1
Exposure time = 2.7 s
Fractions = 36 [usually 1-2 electrons per fractions]
Microscope parameters:
Pixel size = 0.899 A
Magnification = 81,000
Stage tilt = 0 deg
Dose rate = 19.644 e/px/s
Dose rate = 24.28 e/A^2/s
Total dose = 65 e/A^2 [desired dose rate]
Defocus range = -3.5, -3, -2.5, -2, -1.5, -1
C2 aperture = 50 um
Objective aperture= 100 um
Illuminated area = 1.5 um
Energy Filter = YES
Slit width = 20 eV
Grid type = Quantifoil (gold coated) R1.2/1.3 300 Mesh hole size, mesh, etc.
Position 1 of the large dewar Bell Conical: Position 1 of box J2 (Gray)
Prepared on 8/20/2020 by Brian Caldwell
Additional notes:
Copied gain reference files over into the data folder.
Saved EPU settings file.
data/*fractions.mrc