<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11338" schemaVersion="0.63" public="true">
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        <keyDates>
            <depositionDate>2022-12-02</depositionDate>
            <releaseDate>2023-07-07</releaseDate>
            <updateDate>2023-07-07</updateDate>
        </keyDates>
        <title>Cryo-EM SPA dataset for Antibody SKV09 in complex with VEEV VLP (K3 movies/.tif files)</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-2910-0908</authorORCID>
            <firstName>Ryan</firstName>
            <middleName>Gavin</middleName>
            <lastName>Casner</lastName>
            <organization type="academic">Columbia University</organization>
            <street>3227 Broadway</street>
            <townOrCity>New York</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>10027</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-9943-8819</authorORCID>
            <firstName>Lawrence</firstName>
            <lastName>Shapiro</lastName>
            <organization type="academic">Department of Biochemistry, Columbia University</organization>
            <street>3227 Broadway</street>
            <townOrCity>New York</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>10027</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-2910-0908">Casner RG</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">776.7</datasetSize>
        <entryDOI>10.6019/EMPIAR-11338</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-27395</emdbEntry>
            <emdbEntry>EMD-28187</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Sutton MS</author>
                    <author order="2">Pletnev S</author>
                    <author order="3">Callahan V</author>
                    <author order="4">Ko S</author>
                    <author order="5">Tsybovsky Y</author>
                    <author order="6">Bylund T</author>
                    <author authorORCID="0000-0002-2910-0908" order="7">Casner RG</author>
                    <author order="8">Cerutti G</author>
                    <author order="9">Gardner CL</author>
                    <author order="10">Guirguis V</author>
                    <author order="11">Verardi R</author>
                    <author order="12">Zhang B</author>
                    <author order="13">Ambrozak D</author>
                    <author order="14">Beddall M</author>
                    <author order="15">Lei H</author>
                    <author order="16">Yang ES</author>
                    <author order="17">Liu T</author>
                    <author order="18">Henry AR</author>
                    <author order="19">Rawi R</author>
                    <author order="20">Schön A</author>
                    <author order="21">Schramm CA</author>
                    <author order="22">Shen CH</author>
                    <author order="23">Shi W</author>
                    <author order="24">Stephens T</author>
                    <author order="25">Yang Y</author>
                    <author order="26">Florez MB</author>
                    <author order="27">Ledgerwood JE</author>
                    <author order="28">Burke CW</author>
                    <author order="29">Shapiro L</author>
                    <author order="30">Fox JM</author>
                    <author order="31">Kwong PD</author>
                    <author order="32">Roederer M</author>
                    <title>Vaccine elicitation and structural basis for antibody protection against alphaviruses</title>
                    <journal>Cell</journal>
                    <journalAbbreviation>Cell</journalAbbreviation>
                    <country></country>
                    <issue>12</issue>
                    <volume>186</volume>
                    <firstPage>2672</firstPage>
                    <lastPage>2689.e25</lastPage>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.cell.2023.05.019</externalReferences>
                    <externalReferences type="pubmed">37295404</externalReferences>
                    <details>Cryo-EM data for single particle analysis were collected at the Columbia Cryo-EM Facility on a Titan Krios electron microscope operating at 300 kV, equipped with a Gatan K3-BioQuantum direct detection detector and energy filter, using the Leginon software package. Exposures were taken at a magnification of 105,000x (pixel size of 0.83 Å), using a total electron fluence of 46.5 e-/Å2 fractionated over 40 frames with an exposure time of 2.0 s. A random defocus range of -0.8 to -2.0 µm was used.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>K3 Movies (.tif) for SKV09 Antibody + VEEV VLP</name>
        <directory>/data/VEEV_SKV09/movies</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>5595</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>Cryo-EM data for single particle analysis were collected at the Columbia Cryo-EM Facility on a Titan Krios electron microscope operating at 300 kV, equipped with a Gatan K3-BioQuantum direct detection detector and energy filter, using the Leginon software package. Exposures were taken at a magnification of 105,000x (pixel size of 0.83 Å), using a total electron fluence of 46.5 e-/Å2 fractionated over 40 frames with an exposure time of 2.0 s. A random defocus range of -0.8 to -2.0 µm was used.</details>
        <segmentationList/>
        <micrographsFilePattern>data/VEEV_SKV09/movies/*.tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
