<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11315" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-11-21</depositionDate>
            <releaseDate>2022-12-05</releaseDate>
            <updateDate>2022-12-05</updateDate>
        </keyDates>
        <title>CryoEM micrographs of a group II intron retroelement in complex with its structured DNA target (holoRNP)</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-9045-8872</authorORCID>
            <firstName>Anna</firstName>
            <middleName>Marie</middleName>
            <lastName>Pyle</lastName>
            <organization type="academic">Molecular, Cellular and Developmental Biology (MCDB), Yale University</organization>
            <street>260 Whitney Ave</street>
            <townOrCity>New Haven</townOrCity>
            <stateOrProvince>Connecticut</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>06511</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-9045-8872</authorORCID>
            <firstName>Anna</firstName>
            <middleName>Marie</middleName>
            <lastName>Pyle</lastName>
            <organization type="academic">Molecular, Cellular and Developmental Biology (MCDB), Yale University</organization>
            <street>260 Whitney Ave</street>
            <townOrCity>New Haven</townOrCity>
            <stateOrProvince>Connecticut</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>06511</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-0402-6833">Chung KC</author>
            <author authorORCID="0000-0002-9057-0829">Xu LX</author>
            <author authorORCID="0000-0001-9045-8872">Pyle AMP</author>
        </authorsList>
        <datasetSize units="TB">5.6</datasetSize>
        <entryDOI>10.6019/EMPIAR-11315</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-26550</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-0402-6833" order="1">Chung K</author>
                    <author authorORCID="0000-0002-9057-0829" order="2">Xu L</author>
                    <author authorORCID="0000-0001-9702-978X" order="3">Chai P</author>
                    <author authorORCID="0000-0003-2855-2299" order="4">Peng J</author>
                    <author authorORCID="0000-0002-9271-243X" order="5">Devarkar SC</author>
                    <author authorORCID="0000-0001-9045-8872" order="6">Pyle AM</author>
                    <title>Structures of a mobile intron retroelement poised to attack its structured DNA target</title>
                    <journal>Science (New York, N.Y.)</journal>
                    <journalAbbreviation>Science</journalAbbreviation>
                    <country>United States</country>
                    <issue>6620</issue>
                    <volume>378</volume>
                    <firstPage>627</firstPage>
                    <lastPage>634</lastPage>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1126/science.abq2844</externalReferences>
                    <externalReferences type="pubmed">36356138</externalReferences>
                    <details>The micrographs are the average, without any realignment, of 40 raw movie frames (accumulating 50 electron per squared angstrom in a 2 second exposure).</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of a group IIC intron retroelement in complex with its structured DNA target.</name>
        <directory>/data/20210526_Krios_D37_RNP_1</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>10508</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.832</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.832</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned, tilted multi-frame micrographs of a group IIC intron retroelement in complex with its structured DNA target.</name>
        <directory>/data/GroupII_tilted30</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3976</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.832</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.832</pixelHeight>
        </dimensions>
        <details>1321 and 2851 micrographs were collected at 10° and 30° tilt respectively.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
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