REL 2022-11-21 2022-12-19 2022-12-19 CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM. 0000-0002-4814-7517 Anna Maria Steyer European Molecular Biology Laboratory Meyerhofstrasse 1 Heidelberg Baden-Württemberg Germany 69117 0000-0001-8027-1836 Yannick Schwab European Molecular Biology Laboratory Meyerhofstraße 1 Heidelberg Baden-Württemberg Germany 69117 Lleti JMSL Steyer AMS Schwab YS 19.7 10.6019/EMPIAR-11314 FIB-SEM cell Lleti JMSL Steyer AMS Schieber NLS Neumann BN Tischer CT Hilsenstein VH Holtstrom MH Unrau DU Kirmse RK Lucocq JML Pepperkok RP Schwab YS CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM. Journal of Cell Biology JCB United States A1_COPB1 /data/A1 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4260 80.0 1218 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 A2_COPB1 /data/A2 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4254 80.0 1479 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 A3_COPB1 /data/A3 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 3810 80.0 1236 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 A4_COPB1 /data/A4 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4151 80.0 1173 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 A5_COPB1 /data/A5 micrographs - multiframe TIFF TIFF 61 1 UNSIGNED BYTE 3695 80.0 1179 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 B1_COPB1 /data/B1 micrographs - multiframe TIFF TIFF 152 1 UNSIGNED BYTE 2946 80.0 1416 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 B2_COPB1 /data/B2 micrographs - multiframe TIFF TIFF 152 1 UNSIGNED BYTE 3531 80.0 1266 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 B3_COPB1 /data/B3 micrographs - multiframe TIFF TIFF 148 1 UNSIGNED BYTE 4266 80.0 2268 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 B4_COPB1 /data/B4 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 3804 80.0 1488 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 B5_COPB1 /data/B5 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4197 80.0 1326 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 C1_COPB1 /data/C1 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4200 80.0 1353 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 C2_COPB1 /data/C2 micrographs - multiframe TIFF TIFF 122 1 UNSIGNED BYTE 4316 80.0 1221 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 C3_COPB1 /data/C3 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 3981 80.0 1542 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 C4_COPB1 /data/C4 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4319 80.0 1218 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 C5_COPB1 /data/C5 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4313 80.0 1413 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 D1_COPB1 /data/D1 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4099 80.0 1353 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 D2_COPB1 /data/D2 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4200 80.0 1785 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 D3_COPB1 /data/D3 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4319 80.0 1863 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 D4_COPB1 /data/D4 micrographs - multiframe TIFF TIFF 146 1 UNSIGNED BYTE 4376 80.0 1773 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 D5_COPB1 /data/D5 micrographs - multiframe TIFF TIFF 150 1 UNSIGNED BYTE 3872 80.0 1479 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 E1_COPB1 /data/E1 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4203 80.0 1173 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 E2_COPB1 /data/E2 micrographs - multiframe TIFF TIFF 153 1 UNSIGNED BYTE 3324 80.0 2136 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 E3_COPB1 /data/E3 micrographs - multiframe TIFF TIFF 146 1 UNSIGNED BYTE 4203 80.0 1341 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 E4_COPB1 /data/E4 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4099 80.0 1281 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.
 E5_COPB1 /data/E5 micrographs - multiframe TIFF TIFF 147 1 UNSIGNED BYTE 4251 80.0 1428 80.0
Automatically acquired FIB-SEM dataset of GalNAc-T2 cell with voxel size 8x8x200 nm. Knockdown of COPB1 with liquid phase transfection for 48h, afterwards chemically fixed and embedded in Durcupan. Image stack inverted and aligned with SIFT in Fiji.